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Lambda Protein Phosphatase

ABIN2451909 产品详细信息, 供应商: Log in to see
适用
哺乳动物
宿主
大肠杆菌(E. Coli)
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特异性 Release of phosphate groups from phosphorylated serine, threonine, tyrosine and histidine residues in proteins.
产品特性 This product is an intact enzyme of high quality without tag. Lambda Protein Phosphatase (Lambda -PPase) is a Mn+2-dependent protein phosphatase with activity towards phosphorylated serine, threonine, tyrosine and histidine residues. It is the 221 amino-acid product of ORF221 open reading frame on bacteriophage lambda. Lambda -PPase was expressed as a recombinant protein in E.coli and highly purified.
纯度 > 95 % homogeneous protein determined by SDS-PAGE (CBB staining) that contains no detectable protease activity
组件 10 x lambda -PPase Reaction Buffer [500m M Tris-HCl (pH 7.6), 1 M NaCl, 20 mM dithiothreitol, 1 mM EDTA, 0.1 % Brij 35]
10 x Mn2+ (20 mM MnCl2)
UniProt P03772
应用备注 Lambda -PPase can be used to release phosphate groups from phosphorylated serine, threonine, tyrosine and histidine residues in proteins. It should be noted that different proteins are dephosphorylated at different rates. Optimal reaction temperature is 30 °C. Inclusion of protease inhibitor cocktail and shortest incubation time is desired when assays are done with crude samples.
说明

Specific Activity: 400,000 U/mg
One unit is defined as the amount of enzyme that hydrolyzes 1 nmole of p-nitrophenyl phosphate per minute at 30 °C. Unit definition assays are performed with 50 mM p-nitrophenyl phosphate in Lambda-PPase buffer, supplemented with 2 mM MnCl2 in a 50

限制 仅限研究用
状态 Liquid
浓度 400 U/ µL
缓冲液 50 mM HEPES (pH 7.5), 100 mM NaCl, 2 mM dithiothreitol, 0.1 mM MnCl2,0.1 mM EDTA, 50 % glycerol, 0.01 % Brij 35.
储存液 DTT
注意事项 This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Aliquote to avoid repeated freeze-thaw cycles.
储存条件 -80 °C