|应用范围 / 适用||灵长类||人|
|Enzyme Immunoassay (EIA)||1 抗体||2 抗体|
|Flow Cytometry (FACS)||22 抗体||306 抗体|
|Immunohistochemistry (IHC)||12 抗体|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||14 抗体|
|Immunoprecipitation (IP)||5 抗体|
|Western Blotting (WB)||1 抗体|
|Cell Culture (CC)||1 抗体|
|Cytometry by Time of Flight (CyTOF)||5 抗体|
|ELISA (Detection)||1 抗体|
|Functional Studies (Func)||3 抗体|
|Immunochromatography (IC)||1 抗体|
|Immunocytochemistry (ICC)||8 抗体|
|Immunofluorescence (IF)||51 抗体|
|Immunofluorescence (fixed cells) (IF/ICC)||5 抗体|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||1 抗体|
|抗原||Complement Decay-Accelerating Factor (CD55) 抗体|
|适用||人, 灵长类 其他选择|
|标记||This CD55 antibody is un-conjugated 其他选择|
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
CD55抗体详情CD55目标详情 使用细节 贮存及处理 图像
Species reactivity (expected):Primates.
Species reactivity (tested):Human.
|产品特性||Synonyms: Complement decay-accelerating factor|
|纯化方法||Size Exclusion Chromatography.|
|免疫原||HPB-ALL human T cell line.|
CD55目标详情CD55抗体详情 使用细节 贮存及处理 图像 回页首
|别名||CD55 / DAF (CD55 Antibody 摘要)|
|背景||Avoidance by host tissues of attack by autologous complement proteins is dependent in part on the activities of membrane regulatory factors. One molecule involved in this control is a 70 kD glycoprotein termed decay-accelerating factor (DAF), or CD55. Interruption by CD55 of the complement sequence at an early step in activation effectively halts progression of the cascade and prevents consequent cell injury. In man, CD55 is expressed on the plasma membrane of all cell types that are in intimate contact with plasma complement proteins. This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. CD55 also acts as the receptor for echovirus 7 and related viruses(echoviruses 13, 21, 29 and 33). It is attached to the membrane by a GPI-anchor.Synonyms: Complement decay-accelerating factor|
|途径||Complement System, Regulation of Leukocyte Mediated Immunity|
使用细节CD55抗体详情 CD55目标详情 贮存及处理 图像 回页首
Flow Cytometry. Immunoprecipitation. Immunohistochemistry on Paraffin Sections: 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
贮存及处理CD55抗体详情 CD55目标详情 使用细节 图像 回页首
|缓冲液||PBS ~ pH 7.4 containing 15 mM Sodium Azide as preservative.|
|注意事项||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|储存条件||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
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