Binding/Coating Buffer (10X)

ABIN929992 产品详细信息, 供应商: Log in to see
应用范围
ELISA
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产品特性 BBC is a ten times concentrated liquid reagent for coating ELISA plates and other solid phase supports/matrices made of PS or like plastic materials primarily with IgG antibodies. BBC is applicable in both manual and robotic ELISA protocols. 1 Volume of BBC mixed with 9 Volumes of pure deionized water will give 10 Volumes of 50 mM Na-Carbonate/Bicarbonate Buffer pH 9.5 +/-0,03 containing 0,025% 5-Bromo-5-Nitro-1,3- dioxane which is an optimal universal Coating Buffer for antibodies and vast majority of protein antigens (except for very acidic proteins having pI smaller than 5,5) in ELISA and related tests that rely on physical adsorption of biomolecules onto hydrophobic and/or negatively charged plastic surfaces.
过滤 0.2 μm filtered
限制 仅限研究用
状态 Liquid
浓度 10 X
缓冲液 Transparent liquid, 0.2 um filtered. Weak odor characteristic. Supplied as a liquid, pH 9.15, with 5-Bromo-5-nitro-1,3-dioxane as a preservative, 2500 ppm.
储存液 Bromonitrodioxane
注意事项 This product contains Bromonitrodioxane: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C