NEW: 请通过本地经销商订购 4A Biotech Co.,Ltd.

GFP-Booster (Abberior STAR 635N)

ABIN2870918 产品详细信息, 供应商: Log in to see
抗原
  • green fluorescent protein
  • gfp
适用
Aequorea victoria
标记
Abberior STAR 635P
应用范围
Fluorescence Microscopy (FM), Immunofluorescence (IF)
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

原理 With our Booster you reactivate, boost, stabilizate the signals of your fusion proteins.
特异性 GFP-Booster efficiently detects and labels most common GFP derivates. No binding to red fluorescent proteins derived from DsRed can be detected.
产品特性
  • Enhance, stabilize and reactivate your fl uorescent proteins
  • GFP-Booster highly specific for GFP fusion proteins (and derivatives thereof e.g. YFP or Venus)
  • Coupled to bright and photostable chemical dyes
组件 GFP-Trap® coupled to fluorescent dye Abberior STAR 635P
别名 Green Fluorescent Protein
背景 Green fluorescent proteins (GFP) and variants thereof are widely used to study protein localization and dynamics in living cells. However, photo stability and quantum efficiency of GFP are not sufficient for Super-Resolution Microscopy (e.g. 3D-SIM or STED) of fixed samples. In addition, many cell biological methods such as BrdU-staining, EdU-Click-iT™ treatment or Fluorescent In Situ Hybridization result in disruption of the GFP signal.The GFP-Booster_Atto488, a specific GFP-binding protein coupled to the fluorescent dye ATTO 594, reactivates, boosts and stabilizes your GFP signal.
研究领域 Tags/Labels
应用备注 For the immunofluorescence staining of GFP-fusion proteins in fixed cells
ATTO 647N:
Excitation range 615 - 660 nm (λabs= 647 nm)
Emission range 669 - 750 nm (λfl= 669 nm)
说明

Booster are very small, highly specific GFP- or RFP-binding proteins covalently coupled to the superior fluorescent dyes from ATTO-TEC.

实验流程
  • 1. Fixation: 4 % paraformaldehyde (PFA) or 1:10 formalin (37 % formaldehyde, 10-15 % MetOH) in PBS, 10 min., RT.
  • 2. Wash 3x with PBS containing 0.1 % Tween 20 (PBST). Critical: do not let coverslips "dry".
  • 3. Permeabilisation: PBS containing 0.5 % Triton X-100, 5 min., RT. Alternatively permeabilise by incubating in 100 % methanol for 5 min at -20 °C.
  • 4. Wash 2x with PBST.
  • 5. Blocking: 4 % BSA in PBST, 10 min, RT.
  • 6. GFP-Booster incubation: dilute GFP-Booster 1:200 in blocking buffer and incubate 1 h, RT. Note: For multiplexing protocols you can combine GFP-Booster with any other antibody.
  • 7. Wash 3x 5-10 min in PBST.
  • 8. If required counterstain with DNA fluorescent dyes, e.g. DAPI.
  • 9. Before mounting coverslips can be very briefly rinsed in water to prevent salt crystals to form.
  • 10. Mount in VectaShield (Vector Labs) or other mounting media with anti-fading agents and seal mounted coverslips with clear nail polish. Please note: Optimal dilutions/ concentrations should be determined by the end user
限制 仅限研究用
状态 Liquid
浓度 1 mg/mL
缓冲液 PBS, 0.01 % Sodium azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Do not freeze. Protect from light.
储存条件 4 °C
有效期 6 months