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This TMB substrate/stop set is sufficient for 20 microplates Note: The speed of enzymatic colour development depends on the used ELISA system and is influenced by many factors including the specific antibody pair, temperature, quality of the used HRP-conjugate and amount of bound HRP-conjugate. Therefore the sufficient incubation time is very variable und specific for each test system. The development of the colour reaction has to be controlled frequently and should be stopped at an appropriate time point.
Working solution: TMB substrate: 10 mLTMB-B + 0.5 mLTMB-A → 10ml/plate. Mix immediately before use. After mixing, working solution should be colorless or very weak blue. Stop solution: ready-to-use → 5ml/plate