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TMB substrate/STOP ELISA Set

ABIN2746123 产品详细信息, 供应商: Log in to see
应用范围
ELISA
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组件 1 x 10 ml TMB-Solution A
2 x 100 ml TMB-Solution B
1 x 100 ml Stop-solution (ready-to-use)
应用备注 Optimal working dilution should be determined by the investigator.
说明

This TMB substrate/stop set is sufficient for 20 microplates
Note:
The speed of enzymatic colour development depends on the used ELISA system and is influenced by many factors including the specific antibody pair, temperature, quality of the used HRP-conjugate and amount of bound HRP-conjugate. Therefore the sufficient incubation time is very variable und specific for each test system. The development of the colour reaction has to be controlled frequently and should be stopped at an appropriate time point.

实验流程

Working solution: TMB substrate: 10 mLTMB-B + 0.5 mLTMB-A → 10ml/plate. Mix immediately before use. After mixing, working solution should be colorless or very weak blue. Stop solution: ready-to-use → 5ml/plate

限制 仅限研究用
注意事项 The stop solution is an acid solution. TMB-Solution A contain H2O2 and tetramethylbenzidine (TMB). All these compounds are harmful and cause respiratory, skin and eye irritation. Do not swallow any components of the set/kit (R22). Wear face, eye and hand clothing protection when using this material (S36). Keep out of reach of children (S2). Keep away from food, drink and animal feeding stuff (S13).
注意事项 Protect from light!
储存条件 4 °C
有效期 12 months