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Ready-to-use Cell Proliferation Colorimetric Reagent, WST-1

ABIN1304384 产品详细信息, 供应商: Log in to see
适用
哺乳动物
应用范围
Detection (D), Proliferation Assay (ProA)
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原理 The ready-to-use cell proliferation reagent, WST-1 provides a simple and accurate method to measure cell proliferation, which is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. Expansion in the number of viable cells results in an increase in the activity of the mitochondrial dehydrogenases, which in turn leads to increase in the amount of formazan dye formed. The formazan dye produced by viable cells can be quantified by measuring the absorbance at 440 nm. This new method is non-radioactive, rapid and more sensitive than MTT, XTT, or MTS-based assays. The entire assay can be performed in the same microtiter plate and does not require extra steps like washing, harvesting and cell solubilization.
样品类型 Cell Culture Cells, Biological Fluids, Adherent Cell Culture
检测方法 Colorimetric
产品特性 Ready-to-Use Cell Proliferation Reagent, WST-1: Measure Cell Proliferation in response to growth factors, cytokines, mitogens and nutrients etc. in the same microtiter plate. No need to wash, harvest & solubilize cells.
Simple procedure, Fast and convenient, The assay is non-radioactive, rapid and more sensitive than MTT, XTT, or MTS-based assays., The entire assay can be performed in the same microtiter plate and does not require extra steps like washing, harvesting and cell solubilization.

Detection wavelength: 440 nm. The ready-to-use cell proliferation reagent, WST-1 provides a simple and accurate method to measure cell proliferation, which is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. Expansion in the number of viable cells results in an increase in the activity of the mitochondrial dehydrogenases, which in turn leads to increase in the amount of formazan dye formed. The formazan dye produced by viable cells can be quantified by measuring the absorbance at 440 nm. This new method is non-radioactive, rapid and more sensitive than MTT, XTT, or MTS-based assays. The entire assay can be performed in the same microtiter plate and does not require extra steps like washing, harvesting and cell solubilization.
组件 WST-1 Reagent (in electron coupling solution)
背景 The ready-to-use cell proliferation reagent, WST-1 provides a simple and accurate method to measure cell proliferation, which is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. Expansion in the number of viable cells results in an increase in the activity of the mitochondrial dehydrogenases, which in turn leads to increase in the amount of formazan dye formed. The formazan dye produced by viable cells can be quantified by measuring the absorbance at 440 nm. This new method is non-radioactive, rapid and more sensitive than MTT, XTT, or MTS-based assays. The entire assay can be performed in the same microtiter plate and does not require extra steps like washing, harvesting and cell solubilization.
应用备注 Measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. Assessment of physiological mediators and antibodies that inhibit cell growth.
说明

Further details regarding sample type: Cell cultures (adherent and suspension)

限制 仅限研究用
储存条件 -20 °C
有效期 12 months
厂商提供的图像
 image for Ready-to-use Cell Proliferation Colorimetric Reagent, WST-1 (ABIN1304384) Ready-to-use Cell Proliferation Colorimetric Reagent, WST-1
有引用在: Cheng, Chang, Chen, Chen: "Autophagy modulates endoplasmic reticulum stress-induced cell death in podocytes: a protective role." in: Experimental biology and medicine (Maywood, N.J.), Vol. 240, Issue 4, pp. 467-76, 2015 (PubMed).