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Hypersensitive ECL Chemiluminescent Substrate (RTU)

ABIN1113039 产品详细信息, 供应商: Log in to see
应用范围
Western Blotting (WB)
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产品特性 This substrate is an enhanced chemiluminescent substrate with high sensitivity and unique chemiluminescent system. With low background, good stability, sensitive and enhanced signal, this reagent is good for detecting direct and indirect conjugated HRP antibodies and their related antigens, and can detect the target protein sensitively. Catalyzed by HRP, this chemiluminescent substrate can be used to expose X-ray film, and do luminometer detection directly or fluorescence CCD scan. Kit Components Components
样品制备
  1. Proceed the routine SDS-PAGE, transfer membrane and Western Blot steps. 2. Blot membrane: wash membrane with TBS-T twice, 10 min each. Immerse membrane into 5% defatted milk blocking solution, shake by a shaker, and incubate at room temperature for 1 hour. 3. Remove the blocking solution, incubate the membrane in properly diluted primary antibody at room temperature for 2 hours, or at 4℃ overnight on a shaker. Note: Dilute the primary antibody with blocking solution at the antibody manufacturer's recommended dilution. 4. Wash the membrane with 4× or 6× TBS-T for 3 times, 10 min each. Increase the volume of wash buffer, and / or the wash times can reduce the background. Note: Before proceeding Step 4, rinse the membrane with TBS-T simply, the wash efficiency will be increased. 5. Incubate the membrane in properly diluted HRP conjugated secondary antibody at room temperature for 2 hours on a shaker. Note: Dilute the secondary antibody at the antibody manufacturer's recommended dilution. 6. Repeat Step 4 to remove the non-specific conjugation of HRP conjugated secondary antibody. Chongqing Biospes Co., Ltd Product Manual
限制 仅限研究用
状态 Liquid
储存液 Sodium azide, Thimerosal (Merthiolate)
注意事项 This product contains Sodium azide,Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.