Coomassie Brilliant Blue Fast Staining Solution

ABIN1113037 产品详细信息, 供应商: Log in to see
应用范围
Western Blotting (WB)
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产品特性 WB Stripping Buffer is used for the recycle of deproteinized membrane in WB. In WB, after completing the conjugation of the primary and secondary antibodies as well as the later chemiluminescence, sometimes end user has to detect the proteins with stable expression level like Actin and Tubulin, etc. for reference, or test other proteins for comparison. But for this reagent, through dissociating the conjugation of primary, secondary antibodies and antigens, it removes primary and secondary antibodies from the membrane, then end user can reuse the same membrane to detect other proteins. Thus, compare with running a fresh SDS-PAGE gel, this reagent helps to eliminate the loading error and makes the experiment more comparable and accurate. For excluding the frequently-used beta-Mercaptoethanol, this reagent is nontoxic, tasteless and harmless, can be used at room temperature. This reagent is enough for at least 5 times WB detections on the same membrane, and the whole detection can be completed within15-30 min. With the unique component, it is efficient, and has no harm to the proteins. Kit Components Components
样品制备
  1. Wash membrane with TBS-T for 3 times, 10 min each. Immerse it into appropriate volume of WB Stripping Buffer, incubate at room temperature for 15-30 min and shake slowly. (Note: lengthen washing time to 30-60 min for some special antibodies). 2. Take out membrane with tweezer. Elute with TBS-T once and wash for 5 min then. 3. Seal with defatted milk powder or BSA, proceed to the next step.
限制 仅限研究用
状态 Liquid
储存液 Sodium azide, Thimerosal (Merthiolate)
注意事项 This product contains Sodium azide,Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 WB Stripping Buffer (Antibody Stripping Buffer) 100 ml Store at 4 °C for one year, at -20 °C for long term.