RIPA Lysis Buffer (Medium)

ABIN1113013 产品详细信息, 供应商: Log in to see
应用范围
Cell Lysis (CLy), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
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产品特性 1. This RIPA Lysis Buffer is a kind of traditional cell and tissue lysis buffer. The end protein / sample lysed by this buffer can be used for Western Blot and Immunoprecipitation. 2. RIPA (Radio Immunoprecipitation Assay) Lysis Buffer has different formulas, and according to its lysis strength, it can be roughly classified into three categories: strong, medium and weak. 3. RIPA Lysis Buffer (Weak) contains: 50mM Tris (pH 7.4), 150mM NaCl, 1% NP-40, 0.25% sodium deoxycholate, and different kinds of inhibitors (sodium orthovanadate, sodium fluoride, EDTA and leupeptin), which can effectively inhibit the protein degradation. 4. The concentration of the protein which lysed by this buffer can be determined by our BCA Protein Assay Kits (Catalog # BWR1023, BWR1024, BWR1025, BWR1026). For the high concentration of detergent, it is not recommended to use Bradford Protein Assay Kit. Kit Components Components
样品制备

Cultured Cells Sample 1. Pipette proper volume of RIPA Lysis Buffer and mix thoroughly. 2-3 min advanced before use, add PMSF buffer to make its final concentration to1mM. 2. For adherent cells: wash sample with PBS, normal saline or serum-free culture medium to remove culture solution. Add proper volume of RIPA Lysis Buffer, then stroke with pipette until the buffer immerse cells completely. Shake slightly for 5-10 min. After lysis, centrifuge at 10,000-14,000 g for 10 min, then collect the supernatants and move on to the next step. >> Instruction for RIPA usage: for different sizes of cell culture plates <<

限制 仅限研究用
状态 Liquid
储存液 Sodium azide, Thimerosal (Merthiolate)
注意事项 This product contains Sodium azide,Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 RIPA Lysis Buffer (Weak) 100 ml Store at 4 °C for one year