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NP-40 Lysis Buffer

ABIN1113010 产品详细信息, 供应商: Log in to see
应用范围
Western Blotting (WB)
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产品特性 Red Blood Cell Lysis Buffer is specially used for lysing human erythrocytes in single-cell suspensions of peripheral blood and mouse hematopoietic tissues such as spleen. For the major effective ingredient, ammonium chloride, this buffer hardly damage the lymphocyte or other cells with nucleus during the lysis of erythrocytes. Mouse thymus and lymph gland cells do not need this lysis buffer. For the sterile filtration, the obtained blood, tissues or cells can be used for primary culture, cell fusion, nucleic acid or protein extraction and various conventional assay and detection. Kit Components Components
样品制备

Tissues and cells sample 1. Digest the fresh tissues, scatter to suspension by appropriate method. Collect mouse spleen cells to prepare a single-cell suspension. 2. Pellet the cells by centrifugation at 400-500 g at 4°C and aspirate the supernatant. 3. Add Red Blood Cell Lysis Buffer into cell pellet (3-5 times than cell pellet volume), i.e. Add 3-5 ml of Red Blood Cell Lysis Buffer into 1 ml of cell pellet. Stroke slightly and mix thoroughly, and incubate on ice for 4-5 min. During lysis, shake occasionally to promote erythrocytes lysed. (This step can be operated at room temperature or 4°C). 4. Centrifuge at 400-500 g at 4°C for 5 min, remove the red supernatant. 5. Repeat the Step 3 and 4 once if the erythrocyte lysed incompletely. Usually, very small volume of erythrocyte do not affect the follow-up detections. 6. Resuspend the pellet in PBS, HBSS, normal saline or serum-free medium and wash for 1-2 times. Centrifuge at 400-500 g at 4°C for 2-3 min, discard supernatant. The volume of wash buffer should be at least 5 times than cell pellet volume. 7. Perform a cell count after resuspending the pellet. Chongqing Biospes Co., Ltd Product Manual

限制 仅限研究用
状态 Liquid
储存液 Sodium azide, Thimerosal (Merthiolate)
注意事项 This product contains Sodium azide,Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 Red Blood Cell Lysis Buffer 100 ml Stored at 4 °C for one year. Or store at room temperature for 3 months.