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Cell Lysis Buffer for WB & IP

ABIN1113009 产品详细信息, 供应商: Log in to see
应用范围
Western Blotting (WB)
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产品特性 1. Cell Lysis Buffer for Western and IP is a kind of lysis buffer which lyses cells under non-denatured condition. The end cells lysed by this buffer can be used for PAGE, Western Blot, Immunoprecipitation and Co-Immunoprecipitation. 2. This product contains: 20mM Tris (pH 7.5), 150mM NaCl, 1% Triton X-100 and different kinds of inhibitors (sodium pyrophosphate, beta-glycerophosphate, EDTA, Na 3 VO 4 , leupeptin), which can effectively inhibit the protein degradation and maintain the original protein interaction. 3. The concentration of the protein which lysed by this buffer can be determined by our BCA Protein Assay Kits (Catalog # BWR1023, BWR1024, BWR1025, BWR1026). For the high concentration of detergent, it is not recommended to use Bradford Protein Assay Kit. Kit Components Components
样品制备

Cultured Cells Sample 1. Pipette proper volume of the lysis buffer and mix thoroughly. 2-3 min advanced before use, add 2. buffer to make its final concentration to1mM. 2. For adherent cells: wash sample with PBS, normal saline or serum-free culture medium to remove culture solution. Add proper volume of the lysis buffer, then stroke with pipette until the buffer immerse cells completely. Shake slightly for 5-10 min. After lysis, centrifuge at 10,000-14,000 g for 10 min, then collect the supernatants and move on to the next step. >> Instruction for the buffer usage: for different sizes of cell culture plates <<

限制 仅限研究用
状态 Liquid
储存液 Sodium azide, Thimerosal (Merthiolate)
注意事项 This product contains Sodium azide,Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 -20 °C
储存方法 Cell Lysis Buffer for WB & IP 100 ml Store at -20 °C for one year
有效期 12 months