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Protein A Resin

Purif, AC Protein A Agarose beads 90 µm
产品编号 ABIN1536564
发货至: 中国
  • 抗原 See all Protein A products
    Protein A
    适用
    Staphylococcus aureus
    应用范围
    Purification (Purif), Affinity Chromatography (AC)
    原理
    Protein A Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media.
    产品特性
    Protein A Resin can also be used for immunoprecipitation of proteins,protein complexes or antigens. The recombinant protein A ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin is greater than 20 mg rabbit IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.

    Ligand: Recombinant Streptococcal protein A expressed in E. coli
    Number of IgG binding sites per ligand: 5
    MW of ligand: Approximately 34 kDa
    PI of ligand: 5.17
    Degree of substitution: Approximately 2 mg protein A/ml settled resin
    Static binding capacity: >20 mg rabbit IgG/ml settled resin
    Matrix: spherical agarose, 4%
    Average particle size: 90 μm (45-165 μm)
    Bead Ligand
    Protein A
    Bead Matrix
    Agarose beads
    Bead Size
    90 µm
  • 说明

    High temperature heating is not recommended. The agarose melts above 65°C.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    1X PBS containing 20% ethanol
    储存条件
    4 °C
    储存方法
    Store regenerated Protein A Resin in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C. Do not freeze.
    有效期
    18 months
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  • 抗原
    Protein A
    Abstract
    Protein A 产品
    别名
    alpha protein Bead, repB Bead
    背景
    Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding, the Fab region is available for binding antigens. 
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