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人 Stem Cell Array C1

ABIN4955906 产品详细信息, 供应商: Log in to see
适用

实验类型
Sandwich ELISA
应用范围
Antibody Array (AA)
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原理 C-Series Human Stem Cell Array 1 Kit. Semi-quantitative measurement of 15 human stem cell biomarkers. Suitable for all liquid sample types.
样品类型 Plasma, Cell Culture Supernatant, Serum, Cell Lysate, Tissue Lysate
Analytical Method Semi-Quantitative
检测方法 Chemiluminescent
特异性 Alpha-fetoprotein, BMPR-IA (ALK-3), ALK-6 (BMPR-IB), Brachyury (Protein T), CD38, E-Cadherin, GATA4, hCG beta, Nanog, Nestin, OCT-4, PDX-1 (IPF1), SOX17, SOX2, VEGFR3
产品特性
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/mL)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
组件 Antibody Array Membranes
Biotinylated Detection Antibody Cocktail
Blocking Buffer
Wash Buffers 1 and 2
Cell & Tissue Lysis Buffer
Detection Buffers C and D
Plastic Incubation Tray
Protease Inhibitor Cocktail (in select kits)
试剂未包括 Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
应用备注 Perform ALL incubation and wash steps under gentle rotation or rocking motion (~0.5 to 1 cycle/sec) using an orbital shaker or oscillating rocker to ensure complete and even reagent/sample coverage. Rocking/rotating too vigorously may cause foaming or bubbles to appear on the membrane surface which, should be avoided. All washes and incubations should be performed in the Incubation Tray (ITEM 10) provided in the kit. Cover the Incubation Tray with the lid provided during all incubation steps to avoid evaporation and outside debris contamination. Ensure the membranes are completely covered with sufficient sample or reagent volume during each incubation. Avoid forceful pipetting directly onto the membrane, instead, gently pipette samples and reagents into a corner of each well. Aspirate samples and reagents completely after each step by suctioning off excess liquid with a pipette. Tilting the tray so the liquid moves to a corner and then pipetting is an effective method. Optional overnight incubations may be performed for the following step to increase overall spot signal intensities:
- Sample Incubation
- Biotinylated Antibody Cocktail Incubation
- HRP-Streptavidin Incubation
说明

The C-Series arrays feature chemiluminescent signal detection. The antibodies are spotted on nitrocellulose membrane solid supports and are handled in a very similar manner to Western blots.
All C-Series arrays work on the sandwich ELISA principle, utilizing a matched pair of antibodies: an immobilized capture antibody and a corresponding biotinylated detection antibody.

样本量 1 mL
板类型 Membrane
实验流程
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
限制 仅限研究用
注意事项 The antibody printed side of each membrane is marked by a dash (-) or number (#) in the upper left corner. Do not allow membranes to dry out during the experiment or they may become fragile and break OR high and/or uneven background may occur. Grasp membranes by the corners or edges only using forceps. DO NOT touch printed antibody spots.
储存条件 -20 °C
储存方法 For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer a -20°C and all other reagents undiluted at 4°C for no more than 3 months.
厂商提供的图像
 image for Human Stem Cell Array C1 (ABIN4955906) Human Stem Cell Array C1