小鼠 Perforin 1 ELISA Kit

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抗原
Perforin 1 (Pore Forming Protein) (PRF1) ELISA试剂盒
  • PRF1
  • LOC443187
  • perforin
  • prf1
  • FLH2
  • HPLH2
  • P1
  • PFN1
  • PFP
  • Pfn
  • Pfp
  • Prf-1
  • Cyta
  • RATCYTA
  • perforin 1 (pore forming protein)
  • perforin
  • perforin 1-like
  • PRF1
  • LOC443187
  • prf1
  • Prf1
  • LOC100342396
适用
小鼠
其他选择
Kits with alternative reactivity to:
41
14
12
3
3
3
3
2
2
2
2
实验类型
Sandwich ELISA
检测范围
0.625-40 ng/mL
最低检测浓度
0.625 ng/mL
应用范围
ELISA
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产品编号 ABIN1572009
$ 891.06
加上运输费 $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
10.090268 ABIN424746 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Log in to see
10.090268 ABIN4947938 Sandwich ELISA Plasma, Cell Lysate, Cell Culture Supernatant, Serum, Tissue Homogenate, Biological Fluids 0.31-20 ng/mL 0.31 ng/mL Log in to see
10.090268 ABIN1116415 Sandwich ELISA Serum, Plasma, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN5657695 Sandwich ELISA Biological Fluids, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate 0.312 ng/mL - 20 ng/mL 0.312 ng/mL Log in to see
1 ABIN5657693 Sandwich ELISA Biological Fluids, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate 0.156 ng/mL - 10 ng/mL 0.156 ng/mL Log in to see
1 ABIN512021 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 0.5-10 ng/mL 0.5 ng/mL Log in to see
1 ABIN1056977 Serum, Cell Culture Supernatant, Body Fluids, Tissue Homogenate, Plasma Log in to see
1 ABIN5525071 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN2537716 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.31-20 ng/mL 0.31 ng/mL Log in to see
1 ABIN4971972 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 0.625-40 ng/mL 0.625 ng/mL Log in to see
1 ABIN2950241 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN1147371 Log in to see
1 ABIN5069514 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Log in to see
1 ABIN2112837 Competition ELISA Log in to see

General

抗原 Perforin 1 (Pore Forming Protein) (PRF1) ELISA试剂盒
适用 小鼠
Kits with alternative reactivity to:
(41), (14), (12), (3), (3), (3), (3), (2), (2), (2), (2)
实验类型 Sandwich ELISA
检测范围 0.625-40 ng/mL
最低检测浓度 0.625 ng/mL
应用范围 ELISA
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Perforin 1ELISA Kit详情

目标详细情况 使用细节 贮存及处理 图像
原理 The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PRF1 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
样品类型 Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids
检测方法 Colorimetric
Analytical Method Quantitative
特异性 This assay has high sensitivity and excellent specificity for detection of this index.
交叉反应 (详细) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
灵敏度 0.224 ng/mL
组件
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
试剂未包括
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.

目标详细情况

Perforin 1ELISA Kit详情 使用细节 贮存及处理 图像 回页首
抗原
别名 PRF1 (PRF1 ELISA Kit 摘要)
基因ID 18646
UniProt P10820
途径 Apoptosis, Caspase Cascade in Apoptosis

使用细节

Perforin 1ELISA Kit详情 目标详细情况 贮存及处理 图像 回页首
样本量 100 μL
实验时间 1 - 4.5 h
板类型 Pre-coated
实验流程 1. Prepare all reagents, samples and standards
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 µL Stop Solution. Read at 450nm immediately.
实验流程 The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
实验精密度
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV&lt10%
  • Inter-assay: CV&lt12%
限制 仅限研究用

贮存及处理

Perforin 1ELISA Kit详情 目标详细情况 使用细节 图像 回页首
注意事项 The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
注意事项 The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
储存条件 4 °C,-20 °C
储存方法 The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
有效期 12 months

图像

Perforin 1ELISA Kit详情 目标详细情况 使用细节 贮存及处理 回页首
厂商提供的图像
ELISA image for Perforin 1 (Pore Forming Protein) (PRF1) ELISA Kit (ABIN1572009) Perforin 1 (Pore Forming Protein) (PRF1) ELISA Kit