anti-小鼠 PARP1 抗体 for Immunohistochemistry (Frozen Sections)

Recommended PARP1 Antibody (supplied by: Log in to see )

抗原
Poly (ADP-Ribose) Polymerase 1 (PARP1) 抗体
  • BEST:LD21673
  • CG17685
  • CG17696
  • CG17718
  • CG40411
  • D.PARP
  • Dm.pARTa
  • Dmel\\CG40411
  • LD21673.3prime
  • PARP
  • PARP-1
  • PARP1
  • dPARP
  • parp
  • ADPRT
  • ADPRT 1
  • ADPRT1
  • ARTD1
  • PPOL
  • pADPRT-1
  • Adprt
  • Parp-1
  • adprt1
  • padprt-1
  • ppol
  • 5830444G22Rik
  • AI893648
  • Adprp
  • Adprt1
  • C80510
  • parp-1
  • sPARP-1
  • Poly-(ADP-ribose) polymerase
  • poly (ADP-ribose) polymerase 1
  • poly (ADP-ribose) polymerase family, member 1
  • poly ADP-ribose polymerase
  • Parp
  • PARP1
  • Parp1
  • parp1
适用
Fruit Fly (Drosophila melanogaster), Hamster, 人, 小鼠, 大鼠, 非洲爪蟾
385
83
79
18
17
13
9
9
6
5
4
3
2
2
1
1
1
1
1
宿主
小鼠
378
45
1
克隆类型
单克隆
标记
This PARP1 antibody is un-conjugated
25
24
24
24
24
23
应用范围
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
237
231
164
50
43
40
31
22
13
12
5
4
4
3
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

产品编号 ABIN118984
6,551.63 ¥
(含税, 含运费)
来自4A Biotech Co.,Ltd.(本地经销商)
获取报价
中国 北京 101111
No. 88 KeChuang 6th Street
Room 801-803
Beijing Economic Technological Development Area
4A Biotech Co.,Ltd.
Tel 400-7060-959
传真 +86 10 56315212-8813
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1490558 ICC IHC (fro) IHC (p) IP ELISA WB Rabbit IgG AA 1-1014 Log in to see Polyclonal
1 ABIN462120 IHC (fro) IHC (p) IP ELISA WB Mouse IgG1 Log in to see A6-4-12
1 ABIN2476185 IHC (fro) IF IHC (p) IP ELISA WB Mouse IgG1 Log in to see A6-4-12 4
1 ABIN600076 IHC (fro) IHC (p) IP ELISA WB Mouse IgG1 Log in to see

General

抗原 Poly (ADP-Ribose) Polymerase 1 (PARP1) 抗体
适用 Fruit Fly (Drosophila melanogaster), Hamster, 人, 小鼠, 大鼠, 非洲爪蟾
(385), (83), (79), (18), (17), (13), (9), (9), (6), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1)
宿主 小鼠
(378), (45), (1)
克隆类型 (克隆位点)
单克隆   ( )
标记 This PARP1 antibody is un-conjugated
(25), (24), (24), (24), (24), (23)
应用范围 Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(237), (231), (164), (50), (43), (40), (31), (22), (13), (12), (5), (4), (4), (3), (2), (1), (1), (1)
Pubmed 4个引用可参考
Supplier Log in to see

PARP1抗体详情

PARP1目标详情 使用细节 贮存及处理 ProductDetails: References for anti-PARP1 antibody (ABIN118984) 图像
特异性 This antibody recognises poly (ADP-ribose) polymerase (PARP).
交叉反应 (详细) Species reactivity (expected):Xenopus, Drosophila, Hamster, Rat and Mouse.
Species reactivity (tested):Human.
纯化方法 Affinity Chromatography on Protein G.
免疫原 Human PARP. Spleen cells from immunised BALB/c mice were fused with cells of mouse NSO myeloma cell line.
克隆位点 A6-4-12
亚型 IgG1

PARP1目标详情

PARP1抗体详情 使用细节 贮存及处理 ProductDetails: References for anti-PARP1 antibody (ABIN118984) 图像 回页首
抗原
别名 PARP1 (PARP1 Antibody 摘要)
背景 PARP (Poly (ADP-ribose) polymerase) is a 113 kDa nuclear protein which can exist as a homo- or hetero-dimer, and is strongly activated by DNA strand breaks. This protein acts as a molecular ''nick sensor'' and functions in base excision repair, poly(ADPribosyl)ation of acceptor proteins involved in chromatin architecture and DNA metabolism, and participates in protein modification to enhance or repress transcription. PARP also plays a role in other cellular processes, including cell proliferation and differentiation. PARP is ribosylated by PARP2, and during apoptosis, ICE family members, such as caspase 3 and 7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. PARP interacts with proteins in the base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. In addition PARP forms heterodimers with PARP2, and interacts with PARP3.Synonyms: ADPRT, ADPRT 1, NAD(+) ADP-ribosyltransferase 1, PARP-1, PPOL, Poly [ADP-ribose] polymerase 1, Poly[ADP-ribose] synthetase 1
基因ID 142
NCBI登录号 NP_001609
UniProt P09874
途径 Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location

使用细节

PARP1抗体详情 PARP1目标详情 贮存及处理 ProductDetails: References for anti-PARP1 antibody (ABIN118984) 图像 回页首
应用备注 Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. ELISA. Immunoprecipitation. Western Blot: 1/1000 - 1/5000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用

贮存及处理

PARP1抗体详情 PARP1目标详情 使用细节 ProductDetails: References for anti-PARP1 antibody (ABIN118984) 图像 回页首
浓度 1.0 mg/mL
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

ProductDetails: References for anti-PARP1 antibody (ABIN118984)

PARP1抗体详情 PARP1目标详情 使用细节 贮存及处理 图像 回页首
背景

Bie, Yuan, Zhao, Zhang: "Method for estimating the axial intensity derivative in the TIE with higher order intensity derivatives and noise suppression." in: Optics express, Vol. 20, Issue 7, pp. 8186-91, 2012

Harris, Jakob, Taucher-Scholz, Dianov, Becherel, Lavin: "Aprataxin, poly-ADP ribose polymerase 1 (PARP-1) and apurinic endonuclease 1 (APE1) function together to protect the genome against oxidative damage." in: Human molecular genetics, Vol. 18, Issue 21, pp. 4102-17, 2009

Freire, dAdda Di Fagagna, Wu, Pedrazzi, Stagljar, Hickson, Jackson: "Cleavage of the Bloom's syndrome gene product during apoptosis by caspase-3 results in an impaired interaction with topoisomerase IIIalpha." in: Nucleic acids research, Vol. 29, Issue 15, pp. 3172-80, 2001

Krohn, Preis, Prehn: "Staurosporine-induced apoptosis of cultured rat hippocampal neurons involves caspase-1-like proteases as upstream initiators and increased production of superoxide as a main downstream effector." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 20, pp. 8186-97, 1998