LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1) (AA 120-220) 抗体

ABIN968920 产品详细信息, 供应商: Log in to see
抗原
  • PINCH
  • PINCH-1
  • PINCH1
  • Pinch1
  • RGD1560732
  • LIMS3
  • LIMS3L
  • 2310016J22Rik
  • 4921524A02Rik
  • AI507642
  • AU021743
  • AW551584
  • C430041B13Rik
  • Lims1l
  • LIM and senescent cell antigen-like domains 1
  • LIMS1
  • Lims1
抗原表位
AA 120-220
4
4
3
3
1
1
1
适用
小鸡, 犬, 人, 小鼠, 大鼠
53
31
31
4
4
4
4
4
4
3
2
2
2
2
宿主
小鼠
43
11
克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
2
2
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunofluorescence (IF), Western Blotting (WB)
38
19
13
13
9
8
6
5
3
1
1
1
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免疫原 Human PINCH aa. 120-220
克隆位点 49-PINCH
亚型 IgG2a
交叉反应 大鼠, 小鼠
无交叉反应 犬, 小鸡
产品特性 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
纯化方法 The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
别名 PINCH (LIMS1 Antibody 摘要)
背景 Integrins are transmembrane receptors that mediate cell-cell or cell-matrix adhesion. All integrins are heterodimers composed of alpha and beta subunits, which interact with extracellular matrix and cytoskeletal proteins. Signal transduction through intergin receptors may be regulated by integrin-linked kinase (ILK). ILK is a widely expressed Ser/Thr protein kinase that contains four ankyrin-like repeats in the N-terminal region, a phoshoinositide lipid-binding motif at amino acids 180-212, and an integrin binding site at amino acids 293-451. The ankyrin repeats interact with a LIM domain-only protein called PINCH that also binds the adaptor protein, Nck-2. This interaction implicates ILK in growth factor receptor pathways. The PINCH adaptor protein also contains a leucine-rich nuclear export signal and a nuclear localization signal. These two motifs implicate PINCH in nuclear functions, and in Schwann cells PINCH has been found in the nucleus, as well as, cytoplasm and perinuclear regions. Thus, PINCH is an adaptor protein that functions in both integrin signaling and nuclear functions.
分子量 37 kDa
限制 仅限研究用
状态 Liquid
浓度 250 μg/mL
缓冲液 Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 -20 °C
储存方法 Store undiluted at -20°C.
厂商提供的图像
Western Blotting (WB) image for anti-LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1) (AA 120-220) 抗体 (ABIN968920) Western blot analysis of PINCH on K-562 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane ...
有引用在: Campana, Myers, Rearden: "Identification of PINCH in Schwann cells and DRG neurons: shuttling and signaling after nerve injury." in: Glia, Vol. 41, Issue 3, pp. 213-23, 2003 (PubMed).

Tu, Li, Goicoechea, Wu: "The LIM-only protein PINCH directly interacts with integrin-linked kinase and is recruited to integrin-rich sites in spreading cells." in: Molecular and cellular biology, Vol. 19, Issue 3, pp. 2425-34, 1999 (PubMed).

Tu, Li, Wu: "Nck-2, a novel Src homology2/3-containing adaptor protein that interacts with the LIM-only protein PINCH and components of growth factor receptor kinase-signaling pathways." in: Molecular biology of the cell, Vol. 9, Issue 12, pp. 3367-82, 1999 (PubMed).