(3 references)-
- 抗原 See all Phosphoserine products
- Phosphoserine
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Phosphoserine antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF)
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 克隆位点
- 19-pSer
- 亚型
- IgG1
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anti-Phosphoserine antibody
WB, ELISA, IHC 宿主: 小鼠 Monoclonal unconjugated
anti-Phosphoserine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Phosphoserine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal 7A3 unconjugated
anti-Phosphoserine (phosphorylated) antibodyWB 宿主: 小鼠 Monoclonal 5B12 unconjugated
anti-Phosphoserine antibodyWB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphoserine antibodyWB, ELISA, IP 宿主: 小鼠 Monoclonal 2A4 unconjugated
anti-Phosphoserine antibody (FITC)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal FITC
anti-Phosphoserine antibody (Biotin)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal Biotin
anti-Phosphoserine antibody (Atto 488)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 488
anti-Phosphoserine antibody (Atto 594)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 594
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- 应用备注
- Western blot: Typically, 1:2500 is a useful dilution for use in Western Blot.
- 说明
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Related Products: ABIN968887, ABIN967389, ABIN968871
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20° C.
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: "Signal transduction and the control of gene expression." in: Science (New York, N.Y.), Vol. 295, Issue 5556, pp. 813-8, (2002) (PubMed).
: "Signaling through scaffold, anchoring, and adaptor proteins." in: Science (New York, N.Y.), Vol. 278, Issue 5346, pp. 2075-80, (1998) (PubMed).
: "Protein phosphorylation: technologies for the identification of phosphoamino acids." in: Journal of chromatography. A, Vol. 808, Issue 1-2, pp. 23-41, (1998) (PubMed).
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: "Signal transduction and the control of gene expression." in: Science (New York, N.Y.), Vol. 295, Issue 5556, pp. 813-8, (2002) (PubMed).
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- 抗原
- Phosphoserine
- Abstract
- Phosphoserine 产品
- 物质类
- Amino Acid
- 背景
- Protein phosphorylation of serine and threonine residues is critical for the control of protein activity involved in various cellular events. An assortment of Ser/Thr kinases and phosphatases regulate serine and threonine phosphorylation in cell signaling pathways, such as growth factor, cytokine, chemokine, and stress response. Detection of serine and threonine phosphorylation can generally be monitored by antibodies that detect phosphoserine and phosphothreonine. Our clone 19 antibody specifically recognizes phosphoserine modifications on peptides in ELISA, while our clone 22a detects both phosphoserine and phosphothreonine modifications on peptides in ELISA. These antibodies are reported to be useful for Western blot, flow cytometry, and microscopy detection of phosphoserine and phosphothreonine levels.
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