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OCT4 抗体 (AA 252-372)

Name: OCT4 抗体 (AA 252-372)
SKU: ABIN968417
Availability: OutOfStock

POU5F1 适用: 人, 小鼠 WB, IF, BI 宿主: 小鼠 Monoclonal 40-Oct unconjugated

Western Blot analysis of Oct3/4 in human and mouse ES cell lines.

Bottom Row: Immunofluorescent staining of human and mouse ES cell lines.

ES-E14TG2a mouse ES cells (ATCC CRL-1821)

3 images

(6 references)

产品编号 ABIN968417

发货至: 中国

Datasheet as PDF

抗原 See all OCT4 (POU5F1) 抗体

OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
抗原表位
16

10

8

7

6

6

3

3

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 252-372
适用
115

60

30

7

5

5

4

4

4

4

2

2

1

1

1

1

1

1
人, 小鼠
宿主
103

25

1

1
小鼠
Compatible Secondaries
克隆类型
94

35
单克隆
标记
96

5

4

3

3

2

2

2

2

2

2

2

1

1

1

1

1
This OCT4 antibody is un-conjugated
应用范围
108

56

28

21

17

17

15

14

13

11

9

5

3

3

2

2

1

1
Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI)

交叉反应

人

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Mouse Oct3 aa. 252-372 Recombinant Protein

克隆位点

40-Oct

亚型

IgG1 kappa

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anti-POU Class 5 Homeobox 1 (POU5F1) (Internal Region) antibody
POU5F1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) antibody
POU5F1 适用: 人, 猴 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

Bioimaging
1. Seed the cells in appropriate culture medium at an appropriate cell density in an 96-well Imaging Plate , and culture overnight to 48 hours.
2. Remove the culture medium from the wells, wash the wells twice with 100 µl of 1x PBS, and fix the cells by adding 100 µl of fresh 3.7% Formaldehyde in PBS or fixation buffer to each well and incubating for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and wash the wells twice with 100 µl of 1x PBS.
4. Permeabilize the cells by adding 100 µl of 1× Perm/Wash™ buffer to each well and incubating for 30 minutes at RT.
5. Remove the permeabilizer, and wash the wells twice with 100 µl of 1x PBS.
6. Dilute the antibody in Perm/Wash™ buffer, and stain the cells by adding 50 µl of the diluted antibody to each well and incubating for 1 hour at RT.
7. Remove the diluted antibody, and wash the wells three times with 100 µl of 1x PBS.
8. Remove the PBS, dilute the second-step reagent in Perm/Wash™ buffer, and stain the cells by adding 50 µl of the diluted second-step reagent to each well and incubating for 1 hour at RT.
9. Remove the diluted second-step reagent, and wash the wells twice with 100 µl of 1x PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 100 µl of a 2 µg/ml solution of Hoechst 33342 in 1x PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.

说明

Related Products: ABIN968631, ABIN967389

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20° C.
Pan, Thomson: "Nanog and transcriptional networks in embryonic stem cell pluripotency." in: Cell research, Vol. 17, Issue 1, pp. 42-9, (2007) (PubMed).

Nishimoto, Fukushima, Okuda, Muramatsu: "The gene for the embryonic stem cell coactivator UTF1 carries a regulatory element which selectively interacts with a complex composed of Oct-3/4 and Sox-2." in: Molecular and cellular biology, Vol. 19, Issue 8, pp. 5453-65, (1999) (PubMed).

Vigano, Staudt: "Transcriptional activation by Oct-3: evidence for a specific role of the POU-specific domain in mediating functional interaction with Oct-1." in: Nucleic acids research, Vol. 24, Issue 11, pp. 2112-8, (1996) (PubMed).

Yuan, Corbi, Basilico, Dailey: "Developmental-specific activity of the FGF-4 enhancer requires the synergistic action of Sox2 and Oct-3." in: Genes & development, Vol. 9, Issue 21, pp. 2635-45, (1995) (PubMed).

Rosfjord, Scholtz, Lewis, Rizzino: "Phosphorylation and DNA binding of the octamer binding transcription factor Oct-3." in: Biochemical and biophysical research communications, Vol. 212, Issue 3, pp. 847-53, (1995) (PubMed).

Okamoto, Okazawa, Okuda, Sakai, Muramatsu, Hamada: "A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells." in: Cell, Vol. 60, Issue 3, pp. 461-72, (1990) (PubMed).
抗原

OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

别名

Oct-3 (POU5F1 产品)

别名

OCT3 antibody, OCT4 antibody, OTF-3 antibody, OTF3 antibody, OTF4 antibody, Oct-3 antibody, Oct-4 antibody, POU5F1 antibody, gp9 antibody, gp-9 antibody, oct4 antibody, pou2 antibody, POU-2 antibody, cb197 antibody, spg/pou2 antibody, chunp6868 antibody, wu:fd18d06 antibody, NF-A3 antibody, Oct-3/4 antibody, Oct3 antibody, Oct3/4 antibody, Oct4 antibody, Otf-3 antibody, Otf-4 antibody, Otf3 antibody, Otf3-rs7 antibody, Otf3g antibody, Otf4 antibody, POU class 5 homeobox 1 antibody, POU domain, class 5, transcription factor 3 antibody, POU domain, class 5, transcription factor 1 antibody, POU5F1 antibody, pou5f3 antibody, Pou5f1 antibody

背景

Development of a multicellular organism from a single fertilized cell is regulated by the coordinated activity of DNA transcription factors. Oct3/4, a member of the POU family of transcription factors, functions in pluripotent cells of early embryonic stem cell (ES) lines and embryonal carcinomas (EC). Other members of the POU family include Oct1, Oct2, Pit-1, and unc-86. The POU domain, a 150-amino acid region that determines binding specificity, is conserved among these proteins and consists of 3 subdomains: POU-specific A and B subdomains and a homeobox-like subdomain. Oct3/4 is expressed in undifferentiated cells, but is lost as cells are induced to differentiate. Oct3/4 is not expressed in adult tissues. The interaction of Oct3/4 with SOX2, another embryonic transcription factor, produces an active complex that regulates expression of genes such as Nanog, UTF1, and FGF4. Although Oct3/4 is specifically phosphorylated on serine residues, this modification is not required for DNA binding, but may affect its transactivation potential. Thus, Oct3/4 is a transcription factor that plays an important role in determining early steps of embryogenesis and differentiation.
Synonyms: Oct3, OTF3, Oct4, OTF4, POU5F1

分子量

46 kDa

途径

Stem Cell Maintenance