NCF2 抗体 (AA 317-469)
(5 references)-
- 抗原 See all NCF2 抗体
- NCF2 (Neutrophil Cytosolic Factor 2 (NCF2))
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抗原表位
- AA 317-469
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This NCF2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF)
- 交叉反应
- 小鼠
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human p67 [phox] aa. 317-469
- 克隆位点
- 29-p67phox
- 亚型
- IgG2b
- Top Product
- Discover our top product NCF2 Primary Antibody
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anti-Neutrophil Cytosolic Factor 2 (NCF2) (AA 178-526) antibody
NCF2 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Neutrophil Cytosolic Factor 2 (NCF2) (N-Term) antibodyNCF2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Neutrophil Cytosolic Factor 2 (NCF2) (AA 227-526) antibodyNCF2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Neutrophil Cytosolic Factor 2 (NCF2) (AA 355-526) antibodyNCF2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Neutrophil Cytosolic Factor 2 (NCF2) (AA 354-525) antibodyNCF2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Neutrophil Cytosolic Factor 2 (NCF2) antibodyNCF2 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Neutrophil Cytosolic Factor 2 (NCF2) antibodyNCF2 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Neutrophil Cytosolic Factor 2 (NCF2) (AA 1-526) antibodyNCF2 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Neutrophil Cytosolic Factor 2 (NCF2) antibodyNCF2 适用: 人, 小鼠, 大鼠 WB, IHC, IP, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Neutrophil Cytosolic Factor 2 (NCF2) (AA 429-478) antibodyNCF2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 说明
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Related Products: ABIN968584, ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20° C.
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: "Mechanism for phosphorylation-induced activation of the phagocyte NADPH oxidase protein p47(phox). Triple replacement of serines 303, 304, and 328 with aspartates disrupts the SH3 domain-mediated intramolecular interaction in p47(phox), thereby activating" in: The Journal of biological chemistry, Vol. 274, Issue 47, pp. 33644-53, (1999) (PubMed).
: "Phosphorylation of the respiratory burst oxidase subunit p67(phox) during human neutrophil activation. Regulation by protein kinase C-dependent and independent pathways." in: The Journal of biological chemistry, Vol. 272, Issue 27, pp. 17204-8, (1997) (PubMed).
: "NADPH oxidase activity is independent of p47phox in vitro." in: The Journal of biological chemistry, Vol. 271, Issue 37, pp. 22578-82, (1996) (PubMed).
: "Assembly of the phagocyte NADPH oxidase: binding of Src homology 3 domains to proline-rich targets." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 22, pp. 10650-4, (1994) (PubMed).
: "Cloning of a 67-kD neutrophil oxidase factor with similarity to a noncatalytic region of p60c-src." in: Science (New York, N.Y.), Vol. 248, Issue 4956, pp. 727-30, (1990) (PubMed).
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: "Mechanism for phosphorylation-induced activation of the phagocyte NADPH oxidase protein p47(phox). Triple replacement of serines 303, 304, and 328 with aspartates disrupts the SH3 domain-mediated intramolecular interaction in p47(phox), thereby activating" in: The Journal of biological chemistry, Vol. 274, Issue 47, pp. 33644-53, (1999) (PubMed).
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- 抗原
- NCF2 (Neutrophil Cytosolic Factor 2 (NCF2))
- 别名
- p67 phox (NCF2 产品)
- 别名
- MGC81877 antibody, zgc:158405 antibody, noxa2 antibody, p67-phox antibody, p67phox antibody, NCF-2 antibody, NOXA2 antibody, P67-PHOX antibody, P67PHOX antibody, Ncf-2 antibody, neutrophil cytosolic factor 2 antibody, neutrophil cytosolic factor 2 L homeolog antibody, neutrophil cytosol factor 2 antibody, hypothetical protein antibody, NCF2 antibody, ncf2.L antibody, ncf2 antibody, p67phox antibody, VDBG_06643 antibody, PGTG_05816 antibody, LOC100136240 antibody, Ncf2 antibody
- 背景
- The neutrophil respiratory burst oxidase (NADPH-oxidase) generates superoxide and secondary oxygen-derived toxic products in response to bacteria or a variety of soluble stimuli. The active site of this enzyme is located in an integral membrane cytochrome, b558, that consists of the two subunits gp91 [phox] and p21 [phox]. Superoxide production depends on the formation of a complex that includes p67 [phox], p47 [phox], and the GTP-binding protein Rac. Upon activation, these proteins translocate from the cytosol to the membrane where they assemble with b558 and induce oxidase activity. p67 [phox] contains two SH3 domains and binds, via its C-terminal SH3 domain, to the proline rich region of p47 [phox]. This binding allows p67 [phox] to indirectly associate with the oxidase. It is thought that the phosphorylated forms of p67 [phox] and p47 [phox] interact and that the phosphorylation of p67 [phox] is regulated by both PKC-dependent and independent pathways. Although the role of p67 [phox] in electron flow control is poorly understood, it is thought that it regulates the transfer of electrons from NADPH to reduce flavin.
- 分子量
- 67 kDa
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