PRKAR2B 抗体 (AA 1-418)
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- 抗原 See all PRKAR2B 抗体
- PRKAR2B (Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B))
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抗原表位
- AA 1-418
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适用
- 人, 小鼠, 大鼠, 小鸡, 犬
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This PRKAR2B antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
- 交叉反应
- 小鸡, 犬, 小鼠, 大鼠
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human PKA RIIbeta, aa 1-418
- 克隆位点
- 45
- 亚型
- IgG1
- Top Product
- Discover our top product PRKAR2B Primary Antibody
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anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) antibody
PRKAR2B 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (Ser113) antibodyPRKAR2B 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (Internal Region) antibodyPRKAR2B 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (Ser113) antibodyPRKAR2B 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (AA 32-62), (N-Term) antibodyPRKAR2B 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB3253 unconjugated
anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (pSer113) antibodyPRKAR2B 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (pSer113) antibodyPRKAR2B 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (AA 304-413) antibodyPRKAR2B 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (AA 1-418) antibodyPRKAR2B 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (AA 32-62) antibodyPRKAR2B 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS. - 说明
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Related Products: ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20° C.
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: "Regulation of GluR1 by the A-kinase anchoring protein 79 (AKAP79) signaling complex shares properties with long-term depression." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 8, pp. 3044-51, (2002) (PubMed).
: "Phosphodiesterase 4D and protein kinase a type II constitute a signaling unit in the centrosomal area." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 21999-2002, (2001) (PubMed).
: "Mutations in the protein kinase A R1alpha regulatory subunit cause familial cardiac myxomas and Carney complex." in: The Journal of clinical investigation, Vol. 106, Issue 5, pp. R31-8, (2000) (PubMed).
: "Identification, purification, and characterization of subunits of cAMP-dependent protein kinase in human testis. Reverse mobilities of human RII alpha and RII beta on sodium dodecyl sulfate-polyacrylamide gel electrophoresis compared with rat and bovine R" in: The Journal of biological chemistry, Vol. 267, Issue 8, pp. 5374-9, (1992) (PubMed).
: "The two mRNA forms for the type I alpha regulatory subunit of cAMP-dependent protein kinase from human testis are due to the use of different polyadenylation site signals." in: Biochemical and biophysical research communications, Vol. 167, Issue 1, pp. 323-30, (1990) (PubMed).
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: "Regulation of GluR1 by the A-kinase anchoring protein 79 (AKAP79) signaling complex shares properties with long-term depression." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 8, pp. 3044-51, (2002) (PubMed).
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- 抗原
- PRKAR2B (Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B))
- 别名
- PKA RIIbeta (PRKAR2B 产品)
- 别名
- MGC83177 antibody, PRKAR2B antibody, AI451071 antibody, AW061005 antibody, PKARIIbeta antibody, Pkarb2 antibody, RII(beta) antibody, RATDNA antibody, PRKAR2 antibody, RII-BETA antibody, protein kinase, cAMP-dependent, regulatory subunit type II beta S homeolog antibody, protein kinase cAMP-dependent type II regulatory subunit beta antibody, protein kinase, cAMP-dependent, regulatory, type II, beta antibody, protein kinase, cAMP dependent regulatory, type II beta antibody, protein kinase cAMP-dependent type 2 regulatory subunit beta antibody, prkar2b.S antibody, PRKAR2B antibody, prkar2b antibody, Prkar2b antibody
- 背景
- CAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic (C) and regulatory (R). Four regulatory subunits have been identified: RIalpha, RIß, RIIalpha, and RIIß.These subunits define type I and II cAMP-dependent protein kinases. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and type II holoenzymes have three potential C subunits (Calpha, Cß, or Cgamma). Type II PKA can be distinguished by autophosphorylation of the R-subunits, while type I PKA binds Mg/ATP with high affinity. Most cells express both type I and type II PKAs. Although the Ralpha isoforms are ubiquitously expressed, the Rß isoforms are predominant in nervous and adipose tissues. There are indications that the deletion of the gene for PKA RIIß results in lack of long-term potentiation in a select group of hippocampal cells, suggesting an important role for this protein in the neurosciences.
- 分子量
- 53 kDa
- 途径
- Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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