MAP2 抗体 (AA 19-219)
(2 references)-
- 抗原 See all MAP2 抗体
- MAP2 (Microtubule-Associated Protein 2 (MAP2))
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抗原表位
- AA 19-219
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适用
- 人, 小鼠, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This MAP2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
- 交叉反应
- 大鼠, 小鼠
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human MAP2B aa. 19-219
- 克隆位点
- 18-MAP2B
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product MAP2 Primary Antibody
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anti-Microtubule-Associated Protein 2 (MAP2) antibody
MAP2 适用: 人, 小鼠, Pig WB, ELISA, ICC, IHC (p), IP, IHC (fro) 宿主: 小鼠 Monoclonal MT-08 unconjugated
anti-Microtubule-Associated Protein 2 (MAP2) (AA 260-559) antibodyMAP2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Microtubule-Associated Protein 2 (MAP2) antibodyMAP2 适用: Cow WB, IF, ICC 宿主: 小鸡 Polyclonal unconjugated
anti-Microtubule-Associated Protein 2 (MAP2) (AA 350-400) antibodyMAP2 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Microtubule-Associated Protein 2 (MAP2) antibodyMAP2 适用: 人, 小鼠, 大鼠 IF, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Microtubule-Associated Protein 2 (MAP2) antibodyMicroarray verified MAP2 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB573626 unconjugated
anti-Microtubule-Associated Protein 2 (MAP2) (N-Term) antibodyMAP2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Microtubule-Associated Protein 2 (MAP2) (AA 727-985) antibodyMAP2 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Microtubule-Associated Protein 2 (MAP2) (AA 1459-1718) antibodyMAP2 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Microtubule-Associated Protein 2 (MAP2) antibodyMicroarray verified MAP2 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB2355 unconjugated
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- 应用备注
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Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20° C.
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: "A combination of three distinct trafficking signals mediates axonal targeting and presynaptic clustering of GAD65." in: The Journal of cell biology, Vol. 158, Issue 7, pp. 1229-38, (2002) (PubMed).
: "Molecular structure of microtubule-associated protein 2b and 2c from rat brain." in: The Journal of biological chemistry, Vol. 265, Issue 32, pp. 19679-84, (1991) (PubMed).
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: "A combination of three distinct trafficking signals mediates axonal targeting and presynaptic clustering of GAD65." in: The Journal of cell biology, Vol. 158, Issue 7, pp. 1229-38, (2002) (PubMed).
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- 抗原
- MAP2 (Microtubule-Associated Protein 2 (MAP2))
- 别名
- MAP2B (MAP2 产品)
- 别名
- MAP2A antibody, MAP2B antibody, MAP2C antibody, MAP2R antibody, Mtap2 antibody, MAP-2 antibody, Mtap-2 antibody, xmap2 antibody, MGC53131 antibody, a730034c02 antibody, zgc:103474 antibody, zgc:113974 antibody, microtubule associated protein 2 antibody, microtubule-associated protein 2 antibody, microtubule associated protein 2 S homeolog antibody, MAP2 antibody, Map2 antibody, map2.S antibody, map2 antibody
- 背景
- Microtubule-associated proteins (MAPs) play a crucial role in the development and structure of nerve cells. These proteins are important for the assembly and stability of microtubules during neurite outgrowth and for the morphology of neuronal processes, such as dendrites, MAP2, specifically localized in dendrites, has four known isoforms produced by alternative splicing of the transcript. These isoforms, MAPs A, B, C, and D, are expressed at various stages of neuronal development. MAP2B is a 280-kDa protein expressed throughout brain development. It is composed of several highly conserved domains that are flanked by domains with extensive sequence divergence. An N-terminal conserved domain overlaps with a binding domain for the regulatory subunit of the cAMP-dependent kinase II, while a C-terminal conserved domain overlaps with a microtubule-binding domain. Secondary structure prediction suggests that the portion of MAP2B extending from the microtubule surface is composed of a number of alpha-helices separated by small turns which may account for the extended, yet flexible, structure of MAP2B.
- 分子量
- 280 kDa
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