EEA1 抗体 (AA 3-281)
(5 references)-
- 抗原 See all EEA1 抗体
- EEA1 (Early Endosome Antigen 1 (EEA1))
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抗原表位
- AA 3-281
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适用
- 人, 大鼠, 犬, 小鸡
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This EEA1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
- 交叉反应
- 大鼠, 小鸡, 犬
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human EEA1 aa. 3-281
- 克隆位点
- 14-EEA1
- 亚型
- IgG1
- Top Product
- Discover our top product EEA1 Primary Antibody
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anti-Early Endosome Antigen 1 (EEA1) (AA 1312-1411) antibody
EEA1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D4 unconjugated
anti-Early Endosome Antigen 1 (EEA1) (AA 1312-1411) antibodyEEA1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2G2 unconjugated
anti-Early Endosome Antigen 1 (EEA1) (AA 734-875) antibodyEEA1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Early Endosome Antigen 1 (EEA1) (AA 1312-1411) antibodyEEA1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Early Endosome Antigen 1 (EEA1) (C-Term) antibodyEEA1 适用: 人, 小鼠, 大鼠, 犬, Hamster, 马, 兔, Cow, 猴, Pig, 豚鼠, 小鸡, Avian, Cat, 驴, 山羊, 绵羊 WB, IF 宿主: 山羊 Polyclonal unconjugated
anti-Early Endosome Antigen 1 (EEA1) antibodyEEA1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Early Endosome Antigen 1 (EEA1) (AA 1251-1350) antibodyEEA1 适用: 人, Hamster ELISA, ICC, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Early Endosome Antigen 1 (EEA1) (N-Term) antibodyEEA1 适用: 人, 大鼠 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Early Endosome Antigen 1 (EEA1) (N-Term) antibodyEEA1 适用: 人, 小鼠, 大鼠, 犬, 马, 兔, Cow, 豚鼠 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Early Endosome Antigen 1 (EEA1) (AA 2-13) antibodyEEA1 适用: 人, 小鼠, 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
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- 说明
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Related Products: ABIN968545, ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20° C.
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: "Dynamin-dependent transferrin receptor recycling by endosome-derived clathrin-coated vesicles." in: Molecular biology of the cell, Vol. 13, Issue 1, pp. 169-82, (2002) (PubMed).
: "The transmembrane domain of the Alzheimer's beta-secretase (BACE1) determines its late Golgi localization and access to beta -amyloid precursor protein (APP) substrate." in: The Journal of biological chemistry, Vol. 276, Issue 39, pp. 36788-96, (2001) (PubMed).
: "Rab1 recruitment of p115 into a cis-SNARE complex: programming budding COPII vesicles for fusion." in: Science (New York, N.Y.), Vol. 289, Issue 5478, pp. 444-8, (2000) (PubMed).
: "Syntaxin 7 is localized to late endosome compartments, associates with Vamp 8, and Is required for late endosome-lysosome fusion." in: Molecular biology of the cell, Vol. 11, Issue 9, pp. 3137-53, (2000) (PubMed).
: "EEA1, an early endosome-associated protein. EEA1 is a conserved alpha-helical peripheral membrane protein flanked by cysteine "fingers" and contains a calmodulin-binding IQ motif." in: The Journal of biological chemistry, Vol. 270, Issue 22, pp. 13503-11, (1995) (PubMed).
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: "Dynamin-dependent transferrin receptor recycling by endosome-derived clathrin-coated vesicles." in: Molecular biology of the cell, Vol. 13, Issue 1, pp. 169-82, (2002) (PubMed).
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- 抗原
- EEA1 (Early Endosome Antigen 1 (EEA1))
- 别名
- EEA1 (EEA1 产品)
- 背景
- Early endosomes are a major cytoplasmic sorting compartment from which receptors and ligands may be distributed to various sites within the cell. Early endosome antigen 1 (EEA1) is a 180 kDa hydrophilic peripheral membrane protein present in cytosol and membrane fractions. Immunofluorescence studies show that EEA1 colocalizes to early endosomes with transferrin receptor and Rab5, but not with the late endosome-localizing Rab7. EEA1 is predominantly alpha-helical and shares 17-20% sequence identity with the myosins. It contains a calmodulin-binding IQ motif and metal-binding cysteine finger motifs. It is thought that EEA1 is required for vesicular transport of proteins through early endosomes and that these finger motifs are required for this activity. This antibody is routinely tested by western blot analysis.
- 分子量
- 180 kDa
- 途径
- Toll-Like Receptors Cascades
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