Phosphotyrosine 抗体
(5 references)-
- 抗原 See all Phosphotyrosine products
- Phosphotyrosine
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Phosphotyrosine antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 克隆位点
- PY69
- 亚型
- IgG2a
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anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phosphotyrosine antibodyWB, IP, IF, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal E10 unconjugated
anti-Phosphotyrosine antibodyWB, IP, IHC, FACS, ELISA, ICC 宿主: 兔 Monoclonal RM111 unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, FACS, ICC 宿主: 小鼠 Monoclonal P-Tyr-01 unconjugated
anti-Phosphotyrosine antibodyWB, ELISA 宿主: 小鼠 Monoclonal unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal 3F6 unconjugated
anti-Phosphotyrosine antibodyWB, IP, IF, IHC, FACS, ISt, StM 宿主: 小鼠 Monoclonal PY20 unconjugated
anti-Phosphotyrosine antibodyWB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 unconjugated
anti-Phosphotyrosine antibody (HRP)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 HRP
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- 应用备注
- The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- Aqueous buffered solution containing glycerol and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20°C.
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: "Akt mediates sequestration of the beta(2)-adrenergic receptor in response to insulin." in: The Journal of biological chemistry, Vol. 277, Issue 17, pp. 15124-31, (2002) (PubMed).
: "Insulin stimulates phosphorylation of the beta 2-adrenergic receptor by the insulin receptor, creating a potent feedback inhibitor of its tyrosine kinase." in: The Journal of biological chemistry, Vol. 277, Issue 12, pp. 10698-703, (2002) (PubMed).
: "Integrin-induced epidermal growth factor (EGF) receptor activation requires c-Src and p130Cas and leads to phosphorylation of specific EGF receptor tyrosines." in: The Journal of biological chemistry, Vol. 277, Issue 11, pp. 9405-14, (2002) (PubMed).
: "Oncogenic Ki-ras confers a more aggressive colon cancer phenotype through modification of transforming growth factor-beta receptor III." in: The Journal of biological chemistry, Vol. 276, Issue 2, pp. 1555-63, (2001) (PubMed).
: "Monoclonal antibodies to phosphotyrosine." in: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, (1988) (PubMed).
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: "Akt mediates sequestration of the beta(2)-adrenergic receptor in response to insulin." in: The Journal of biological chemistry, Vol. 277, Issue 17, pp. 15124-31, (2002) (PubMed).
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- 抗原
- Phosphotyrosine
- Abstract
- Phosphotyrosine 产品
- 物质类
- Amino Acid
- 背景
- Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain cellular protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine provide one of the best tools for the detection and characterization of phosphotyrosine proteins.
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