电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

GNB2L1 抗体 (AA 113-317)

GNB2L1 适用: 人, 小鼠, 大鼠, Cow, 犬, 小鸡, Frog WB, IHC, IP, BI 宿主: 小鼠 Monoclonal 20-RACK1 unconjugated
产品编号 ABIN967797
发货至: 中国
  • 抗原 See all GNB2L1 抗体
    GNB2L1 (Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1))
    抗原表位
    • 8
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 113-317
    适用
    • 54
    • 30
    • 30
    • 8
    • 8
    • 7
    • 7
    • 7
    • 6
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    人, 小鼠, 大鼠, Cow, 犬, 小鸡, Frog
    宿主
    • 56
    • 1
    • 1
    小鼠
    克隆类型
    • 47
    • 11
    单克隆
    标记
    • 30
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This GNB2L1 antibody is un-conjugated
    应用范围
    • 40
    • 22
    • 20
    • 9
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), BioImaging (BI)
    交叉反应
    人, 牛, 小鸡, 犬, Frog, 小鼠
    产品特性
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    6. Triton is a trademark of the Dow Chemical Company.
    纯化方法
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    免疫原
    Rat RACK1 aa 113-317
    克隆位点
    20-RACK1
    亚型
    IgM
    Top Product
    Discover our top product GNB2L1 Primary Antibody
  • 应用备注
    Bioimaging
    1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
    2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
    3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
    4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
    5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
    6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
    7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
    8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
    9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
    10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
    11. View and analyze the cells on an appropriate imaging instrument.
    说明

    Related Products: ABIN968537

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    250 μg/mL
    缓冲液
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Store undiluted at -20°C.
  • Birikh, Sklan, Shoham, Soreq: "Interaction of "readthrough" acetylcholinesterase with RACK1 and PKCbeta II correlates with intensified fear-induced conflict behavior." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 1, pp. 283-8, (2003) (PubMed).

    Liedtke, Yun, Kyle, Wang: "Protein kinase C epsilon-dependent regulation of cystic fibrosis transmembrane regulator involves binding to a receptor for activated C kinase (RACK1) and RACK1 binding to Na+/H+ exchange regulatory factor." in: The Journal of biological chemistry, Vol. 277, Issue 25, pp. 22925-33, (2002) (PubMed).

    Chang, Chiang, Cartwright: "The interaction of Src and RACK1 is enhanced by activation of protein kinase C and tyrosine phosphorylation of RACK1." in: The Journal of biological chemistry, Vol. 276, Issue 23, pp. 20346-56, (2001) (PubMed).

    Smart, Ying, Anderson: "Hormonal regulation of caveolae internalization." in: The Journal of cell biology, Vol. 131, Issue 4, pp. 929-38, (1996) (PubMed).

    Ron, Chen, Caldwell, Jamieson, Orr, Mochly-Rosen: "Cloning of an intracellular receptor for protein kinase C: a homolog of the beta subunit of G proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 3, pp. 839-43, (1994) (PubMed).

  • 抗原
    GNB2L1 (Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1))
    别名
    RACK1 (GNB2L1 产品)
    别名
    MGC53289 antibody, GNB2L1 antibody, AL033335 antibody, GB-like antibody, Gnb2-rs1 antibody, Rack1 antibody, p205 antibody, RACK1 antibody, H12.3 antibody, HLC-7 antibody, PIG21 antibody, rack1 antibody, wu:fb80d08 antibody, wu:fk65d12 antibody, gnb2-rs1 antibody, h12.3 antibody, hlc-7 antibody, pig21 antibody, C12-3 antibody, c12.3 antibody, guanine nucleotide binding protein, beta 2, related sequence 1 antibody, receptor for activated C kinase 1 antibody, guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1 antibody, receptor for activated C kinase 1 L homeolog antibody, Guanine nucleotide-binding protein subunit beta-2-like 1 antibody, gnb2l1 antibody, RACK1 antibody, Rack1 antibody, rack1.L antibody, rack-1 antibody
    背景
    Several proteins which specifically bind to PKC have been classified as RACKs (receptors for activated C-kinase). RACK1 was cloned from a rat brain cDNA expression library by screening for proteins that bind PKC in the presence of phosphatidylserine, diacylglycerol, and calcium in a PKC overlay assay. By sequence homology, RACK1 appears to belong to a superfamily that includes the ß subunit of G proteins. All of these proteins contain five to eight internal repeat elements known as WD40 motifs, which appear to have a role in protein-protein interactions. In addition, RACK1 contains two short sequences homologous to a PKC-binding sequence identified in Annexin I and in the brain PKC inhibitor KCIP. The binding of RACK1 to PKC is dose-dependent and occurs at a site on PKC that is distinct from the catalytic domain, indicating that RACK1 is not a PKC substrate.
    分子量
    36 kDa
    途径
    cAMP Metabolic Process, Positive Regulation of Endopeptidase Activity
You are here:
客服