(6 references)-
- 抗原 See all Phosphotyrosine products
- Phosphotyrosine
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Phosphotyrosine antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 克隆位点
- PY20
- 亚型
- IgG2b
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anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phosphotyrosine antibodyWB, IP, IF, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal E10 unconjugated
anti-Phosphotyrosine antibodyWB, IP, IHC, FACS, ELISA, ICC 宿主: 兔 Monoclonal RM111 unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, FACS, ICC 宿主: 小鼠 Monoclonal P-Tyr-01 unconjugated
anti-Phosphotyrosine antibody (FITC)WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal FITC
anti-Phosphotyrosine antibody (Atto 488)WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal Atto 488
anti-Phosphotyrosine antibody (Atto 594)WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal Atto 594
anti-Phosphotyrosine antibody (Atto 390)WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal Atto 390
anti-Phosphotyrosine antibodyWB, ELISA 宿主: 小鼠 Monoclonal unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
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- 应用备注
- The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes. This antibody is routinely tested by western blot analysis. Other applications were tested during antibody development only or reported in the literature.
- 说明
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Related Products: ABIN968534, ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- Aqueous buffered solution containing glycerol and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20° C.
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: "MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation." in: Immunity, Vol. 47, Issue 1, pp. 51-65.e7, (2017) (PubMed).
: "The rasGAP-binding protein, Dok-1, mediates activin signaling via serine/threonine kinase receptors." in: The EMBO journal, Vol. 21, Issue 7, pp. 1684-94, (2002) (PubMed).
: "The intrinsic ability of AFAP-110 to alter actin filament integrity is linked with its ability to also activate cellular tyrosine kinases." in: Oncogene, Vol. 20, Issue 45, pp. 6607-16, (2001) (PubMed).
: "Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions." in: Cytometry, Vol. 39, Issue 4, pp. 250-9, (2000) (PubMed).
: "Evidence that Argos is an antagonistic ligand of the EGF receptor." in: Oncogene, Vol. 19, Issue 31, pp. 3560-2, (2000) (PubMed).
: "Monoclonal antibodies to phosphotyrosine." in: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, (1988) (PubMed).
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: "MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation." in: Immunity, Vol. 47, Issue 1, pp. 51-65.e7, (2017) (PubMed).
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- 抗原
- Phosphotyrosine
- Abstract
- Phosphotyrosine 产品
- 物质类
- Amino Acid
- 背景
- Phosphorylation of specific tyrosine residues is the result of of activation or stimulation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain cellular protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine provide one of the best tools for the detection and characterization of phosphotyrosine proteins. Technical Note: The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes. This antibody is routinely tested by western blot analysis.
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