电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

Nanog 抗体

NANOG 适用: 人 WB, BI 宿主: 小鼠 Monoclonal N31-355 unconjugated
产品编号 ABIN967670
发货至: 中国
  • 抗原 See all Nanog (NANOG) 抗体
    Nanog (NANOG) (Nanog Homeobox (NANOG))
    适用
    • 138
    • 59
    • 23
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    宿主
    • 128
    • 15
    • 3
    • 1
    小鼠
    克隆类型
    • 123
    • 24
    单克隆
    标记
    • 77
    • 10
    • 9
    • 9
    • 8
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    This Nanog antibody is un-conjugated
    应用范围
    • 116
    • 65
    • 48
    • 47
    • 26
    • 26
    • 24
    • 18
    • 18
    • 17
    • 8
    • 7
    • 5
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), BioImaging (BI)
    品牌
    BD Pharmingen™
    产品特性
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Triton is a trademark of the Dow Chemical Company.
    4. Alexa Fluor is a registered trademark of Molecular Probes, Inc., Eugene, OR.
    5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    纯化方法
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    免疫原
    Human Nanog Recombinant Protein
    克隆位点
    N31-355
    亚型
    IgG1 kappa
    Top Product
    Discover our top product NANOG Primary Antibody
  • 应用备注
    Bioimaging:
    1. Seed the cells in appropriate culture medium at an appropriate cell density in an 96-well Imaging Plate , and culture overnight to 48 hours.
    2. Remove the culture medium from the wells, and wash (one to two times) with 100 myl of 1× PBS.
    Fix the cells by adding 100 µl of fresh 3.7% Formaldehyde in PBS or fixation buffer to each well and incubating for 10 minutes at room temperature (RT).
    4. Remove the fixative from the wells, and wash the wells (one to two times) with 100 myl of 1× PBS.
    5. Permeabilize the cells using either cold methanol (a), Triton™ X-100 (b), or Saponin (c): a. Add 100 µl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. b. Add 100 µl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT. c. Add 100 µl of 1× Perm/Wash buffer to each well and incubate for 15 to 30 minutes at RT. Continue to use 1× Perm/Wash buffer for all subsequent wash and dilutions steps.
    6. Remove the permeabilization buffer from the wells, and wash one to two times with 100 myl of appropriate buffer (either 1× PBS or 1× Perm/Wash buffer, see step 5.c.).
    7. Optional blocking step: Remove the wash buffers, and block the cells by adding 100 µl of blocking buffer or 3% FBS in appropriate dilution buffer to each well and incubating for 15 to 30 minutes at RT.
    8. Dilute the antibody to its optimal working concentration in appropriate dilution buffer. Titrate purified (unconjugated) antibodies and second-step reagents to determine the optimal concentration. If using a Bioimaging Certified antibody conjugate, dilute it 1:10.
    9. Add 50 µl of diluted antibody per well and incubate for 60 minutes at RT. Incubate in the dark if using fluorescently labeled antibodies.
    10. Remove the antibody, and wash the wells three times with 100 myl of wash buffer. An optional detergent wash (100 myl of 0.05% Tween in 1× PBS) can be included prior to the regular wash steps.
    11. If the antibody being used is fluorescently labeled, then move to step 12. Otherwise, if using a purified unlabeled antibody, repeat steps 8 to 10 with a fluorescently labeled second-step reagent to detect the purified antibody.
    12. After the final wash, counter-stain the nuclei by adding 100 ml of a 2 mg/ml solution of Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
    13. View and analyze the cells on an appropriate imaging instrument.
    说明

    Related Products: ABIN967389

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store undiluted at 4°C.
  • Pan, Thomson: "Nanog and transcriptional networks in embryonic stem cell pluripotency." in: Cell research, Vol. 17, Issue 1, pp. 42-9, (2007) (PubMed).

    Yu, Vodyanik, Smuga-Otto, Antosiewicz-Bourget, Frane, Tian, Nie, Jonsdottir, Ruotti, Stewart, Slukvin, Thomson: "Induced pluripotent stem cell lines derived from human somatic cells." in: Science (New York, N.Y.), Vol. 318, Issue 5858, pp. 1917-20, (2007) (PubMed).

    Sun, Li, Yang, Rao, Zhan: "Mechanisms controlling embryonic stem cell self-renewal and differentiation." in: Critical reviews in eukaryotic gene expression, Vol. 16, Issue 3, pp. 211-31, (2006) (PubMed).

    Suzuki, Raya, Kawakami, Morita, Matsui, Nakashima, Gage, Rodríguez-Esteban, Izpisúa Belmonte: "Nanog binds to Smad1 and blocks bone morphogenetic protein-induced differentiation of embryonic stem cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 27, pp. 10294-9, (2006) (PubMed).

    Ezeh, Turek, Reijo, Clark: "Human embryonic stem cell genes OCT4, NANOG, STELLAR, and GDF3 are expressed in both seminoma and breast carcinoma." in: Cancer, Vol. 104, Issue 10, pp. 2255-65, (2005) (PubMed).

    Chambers, Colby, Robertson, Nichols, Lee, Tweedie, Smith: "Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells." in: Cell, Vol. 113, Issue 5, pp. 643-55, (2003) (PubMed).

    Mitsui, Tokuzawa, Itoh, Segawa, Murakami, Takahashi, Maruyama, Maeda, Yamanaka: "The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells." in: Cell, Vol. 113, Issue 5, pp. 631-42, (2003) (PubMed).

  • 抗原
    Nanog (NANOG) (Nanog Homeobox (NANOG))
    别名
    Nanog (NANOG 产品)
    别名
    2410002E02Rik antibody, ENK antibody, ecat4 antibody, NANOG antibody, hacp antibody, wu:fd19e04 antibody, wu:fd20a07 antibody, zgc:193933 antibody, Nanog homeobox antibody, nanog homeobox antibody, Nanog antibody, NANOG antibody, nanog antibody
    背景
    The N31-355 monoclonal antibody reacts with human Nanog (named for Tir Na Nog, the land of the ever-young of Celtic mythology), which is a homeobox transcription factor required for the maintenance of the undifferentiated state of pluripotent stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF (Leukemia Inhibitory Factor) and BMP (Bone Morphogenic Protein). When Nanog expression is down-regulated, cell differentiation can proceed. Proteins that regulate Nanog expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53. Nanog is one of the factors that can contribute to reprogramming of differentiated cells to an induced pluripotent stem cell state.
    分子量
    36-37 kDa
    途径
    Stem Cell Maintenance
You are here:
客服