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CDK4 抗体

CDK4 适用: 人, 小鼠 WB, IP, IHC (f) 宿主: 小鼠 Monoclonal DCS-156 unconjugated
产品编号 ABIN967647
发货至: 中国
  • 抗原 See all CDK4 抗体
    CDK4 (Cyclin-Dependent Kinase 4 (CDK4))
    适用
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    人, 小鼠
    宿主
    • 108
    • 31
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    • 1
    小鼠
    克隆类型
    • 107
    • 34
    单克隆
    标记
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    This CDK4 antibody is un-conjugated
    应用范围
    • 119
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    Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    品牌
    BD Pharmingen™
    交叉反应
    小鼠
    产品特性
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    纯化方法
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    免疫原
    Recombinant Human Cdk4
    克隆位点
    DCS-156
    亚型
    IgG1 kappa
    Top Product
    Discover our top product CDK4 Primary Antibody
  • 应用备注
    Applications include western blot analysis (0.5-2.0 µg/ml) and immunoprecipitation (1.0 µg/one million cells). The antibody has also been used for immunohistochemistry of formalin-fixed, paraffin-embedded tissue sections, but this application is not routinely tested. HeLa (ATCC CCL 2), 293 (ATCC CRL 1573), or NIH/3T3 (ATCC CRL 1658) cells are suggested as positive controls.
    说明

    Related Products: ABIN967389, ABIN968535

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store undiluted at 4°C.
  • Johnson, Walker: "Cyclins and cell cycle checkpoints." in: Annual review of pharmacology and toxicology, Vol. 39, pp. 295-312, (1999) (PubMed).

    Matsushime, Ewen, Strom, Kato, Hanks, Roussel, Sherr: "Identification and properties of an atypical catalytic subunit (p34PSK-J3/cdk4) for mammalian D type G1 cyclins." in: Cell, Vol. 71, Issue 2, pp. 323-34, (1992) (PubMed).

  • 抗原
    CDK4 (Cyclin-Dependent Kinase 4 (CDK4))
    别名
    Cdk4 (CDK4 产品)
    别名
    zgc:153726 antibody, CMM3 antibody, PSK-J3 antibody, Crk3 antibody, 8-6 antibody, CDK4 antibody, CDK4/6 antibody, CG5072 antibody, Cdk4/6 antibody, DmCdk4 antibody, Dmel\\CG5072 antibody, Pk53C antibody, Pk?7 antibody, cdk antibody, cdk4 antibody, cdk4/6 antibody, dCdk4 antibody, l(2)05428 antibody, l(2)0671 antibody, l(2)k06503 antibody, l(2)s4639 antibody, l(2)sh0671 antibody, xcdk4 antibody, cyclin-dependent kinase 4 antibody, cyclin dependent kinase 4 antibody, Cyclin-dependent kinase 4 antibody, cyclin-dependent kinase 4 L homeolog antibody, cdk4 antibody, CDK4 antibody, Cdk4 antibody, cdk4.L antibody
    背景
    Cyclins, cyclin-dependent kinases (Cdks), and cyclin-dependent kinase inhibitors (CdkIs) are essential for cell-cycle control in eukarytotes. Cyclins, regulatory subunits, bind to cyclin-dependent kinases (Cdks), catalytic subunits, to form active cyclin-Cdk complexes. Cdk subunits by themselves are inactive and binding to a cyclin is required for their activity. Cyclins A, B1, D and E undergo periodic synthesis and degradation, thereby providing a mechanism to regulate Cdk activity throughout the cell cycle. Additionally, Cdk activity is further regulated by activating and inhibitory phosphorylations, and small proteins (p15, p16, p18, p19, p21 and p27), called inhibitors of Cdk activity, that bind to cyclins, Cdks, or cyclin- Cdk complexes. Cdk4 was originally called PSK-J3, and following demonstration of its association with D-type cyclins, became known as Cdk4. D-type cyclins also associate with Cdks 2 and 5, although Cdk4 appears to be the most abundant partner. The D-type cyclins (D1, D2, and D3) are expressed in response to growth factors or mitogens, and rapidly degrade when mitogens are withdrawn. D cyclins appear to promote G0 to G1 transitions and the rate of G1 progression. For example, cyclin D-Cdk4 and cyclin D-Cdk6 complexes phosphorylate the retinoblastoma protein (Rb) which removes the G1 phase block caused by underphosphorylated Rb. Cdk4 has a molecular weight of ~32 kD. Clone DCS-156 recognizes human and mouse Cdk4. A recombinant protein fragment from the C-terminal end of human Cdk4 was used as immunogen.
    分子量
    32 kDa
    途径
    Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size
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