(6 references)-
- 抗原 See all MCL-1 (MCL1) 抗体
- MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This MCL-1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP)
- 品牌
- BD Pharmingen™
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Purified recombinant human Mcl-1 lacking the C-terminal hydrophobic domain
- 克隆位点
- 22-Mcl
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product MCL1 Primary Antibody
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anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 2-307) antibody
MCL1 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (Center) antibodyMCL1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 1-350) antibodyMCL1 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) antibodyMCL1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC, IP 宿主: 兔 Monoclonal unconjugated
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 19-244) antibodyMCL1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) antibodyMCL1 适用: 人, 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 120-220) antibodyMCL1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) antibodyMCL1 适用: 人 WB, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 8C6 unconjugated
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 191-226) antibodyMCL1 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 6-328) antibodyMCL1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- The antibody is tested by Western blot on K-562 cell lysate (ATCC CCL-243). Other applications include immunoprecipitation (1-2 µg/ml).
- 说明
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Related Products: ABIN968586, ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4° C.
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: "BCL-2 family members and the mitochondria in apoptosis." in: Genes & development, Vol. 13, Issue 15, pp. 1899-911, (1999) (PubMed).
: "The antiapoptotic gene mcl-1 is up-regulated by the phosphatidylinositol 3-kinase/Akt signaling pathway through a transcription factor complex containing CREB." in: Molecular and cellular biology, Vol. 19, Issue 9, pp. 6195-206, (1999) (PubMed).
: "The Bcl-2 protein family: arbiters of cell survival." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1322-6, (1998) (PubMed).
: "The intracellular distribution and pattern of expression of Mcl-1 overlap with, but are not identical to, those of Bcl-2." in: The Journal of cell biology, Vol. 128, Issue 6, pp. 1173-84, (1995) (PubMed).
: "MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3516-20, (1993) (PubMed).
: "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death." in: Cell, Vol. 74, Issue 4, pp. 609-19, (1993) (PubMed).
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: "BCL-2 family members and the mitochondria in apoptosis." in: Genes & development, Vol. 13, Issue 15, pp. 1899-911, (1999) (PubMed).
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- 抗原
- MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
- 别名
- Mcl-1 (MCL1 产品)
- 别名
- BCL2L3 antibody, EAT antibody, MCL1-ES antibody, MCL1L antibody, MCL1S antibody, Mcl-1 antibody, TM antibody, bcl2-L-3 antibody, mcl1/EAT antibody, AW556805 antibody, mcl-1 antibody, fb72f02 antibody, wu:fb70a08 antibody, wu:fb72f02 antibody, zfMLP1 antibody, zfMcl-1a antibody, zgc:109835 antibody, zgc:109763 antibody, E3 antibody, E3-2 antibody, RNE3 antibody, RNU107 antibody, SNORA63A antibody, elF-4AII antibody, MCL1, BCL2 family apoptosis regulator antibody, myeloid cell leukemia sequence 1 antibody, MCL1, BCL2 family apoptosis regulator a antibody, MCL1, BCL2 family apoptosis regulator b antibody, small nucleolar RNA, H/ACA box 63 antibody, MCL1 antibody, Mcl1 antibody, mcl1a antibody, mcl1b antibody, SNORA63 antibody
- 背景
- The Bcl-2 family of proteins plays a pivotal role in determining the life and death of a cell. Members of the Bcl-2 family all possess at least one of four conserved Bcl-2 homology domains (BH1-BH4) which mediate protein-protein interactions. They can be divided into molecules that have either an anti-apoptotic or pro-apoptotic function. The ratio between these two groups of molecules can help determine whether a cell lives or dies. The majority of anti-apoptotic family members contain common BH1-BH4 domains and are most similar to Bcl-2. Mcl-1 is a member of the anti-apoptotic Bcl-2 group of proteins, including Bcl-x, Bcl-w, A1/Bfl-1, Boo/Diva, and Nr-13. Mcl-1 was cloned from the ML-1 human myeoblastic leukemia cell line. Mcl-1 localizes to the mitochrondria, like Bcl-2, but also associates with membranes via its C-terminal hydrophobic domain. Further studies have shown that Mcl-1 is upregulated in response to the GM-CSF and IL-3 cytokine signaling pathways and that this regulation of Mcl-1 is controlled at the level of transcription. Mcl-1 is detected at approximately 40 kDa in SDS/PAGE.
- 分子量
- 40 kDa
- 途径
- MAPK Pathway
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