(9 references)-
- 抗原 See all CD35 (CR1) 抗体
- CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This CD35 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Blocking Reagent (BR)
- 品牌
- BD Pharmingen™
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Purified mouse CR1
- 克隆位点
- 8C12
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product CR1 Primary Antibody
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anti-Complement Component Receptor 1 (CD35) (CR1) (AA 1780-2039) antibody
CR1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人 IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) (AA 420-550) antibodyCR1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人, Non-Human Primate FACS, WB, IHC (p), IP, IHC (fro), ICC 宿主: 小鼠 Monoclonal E11 unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibody (FITC)CR1 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal E11 FITC
anti-Complement Component Receptor 1 (CD35) (CR1) (AA 901-1095) antibodyCR1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人 IHC, ELISA 宿主: 兔 Monoclonal 9A9 unconjugated Recombinant Antibody
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人 FACS, DNA Mic 宿主: 小鼠 Monoclonal E11 unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人, Baboon, Cynomolgus, 猕猴 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal E11 unconjugated
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- 说明
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Related Products: ABIN967384
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4° C.
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: "Dependence of germinal center B cells on expression of CD21/CD35 for survival." in: Science (New York, N.Y.), Vol. 280, Issue 5363, pp. 582-5, (1998) (PubMed).
: "Expression of the murine CD21 gene is regulated by promoter and intronic sequences." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 10, pp. 4758-68, (1997) (PubMed).
: "Markedly impaired humoral immune response in mice deficient in complement receptors 1 and 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 8, pp. 3357-61, (1996) (PubMed).
: "Disruption of the Cr2 locus results in a reduction in B-1a cells and in an impaired B cell response to T-dependent antigen." in: Immunity, Vol. 4, Issue 3, pp. 251-62, (1996) (PubMed).
: "Murine macrophages lack expression of the Cr2-145 (CR2) and Cr2-190 (CR1) gene products." in: European journal of immunology, Vol. 23, Issue 11, pp. 3037-42, (1993) (PubMed).
: "Two functionally different follicular dendritic cells in secondary lymphoid follicles of mouse spleen, as revealed by CR1/2 and FcR gamma II-mediated immune-complex trapping." in: Immunology, Vol. 80, Issue 1, pp. 34-9, (1993) (PubMed).
: "Characterization of murine complement receptor type 2 and its immunological cross-reactivity with type 1 receptor." in: International immunology, Vol. 2, Issue 7, pp. 651-9, (1991) (PubMed).
: "In vivo inhibition of the antibody response by a complement receptor-specific monoclonal antibody." in: The Journal of experimental medicine, Vol. 172, Issue 2, pp. 665-8, (1990) (PubMed).
: "The murine complement receptor gene family. IV. Alternative splicing of Cr2 gene transcripts predicts two distinct gene products that share homologous domains with both human CR2 and CR1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 9, pp. 3581-91, (1990) (PubMed).
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: "Dependence of germinal center B cells on expression of CD21/CD35 for survival." in: Science (New York, N.Y.), Vol. 280, Issue 5363, pp. 582-5, (1998) (PubMed).
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- 抗原
- CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))
- 别名
- CD35 (CR1) (CR1 产品)
- 别名
- C3BR antibody, C4BR antibody, CD35 antibody, KN antibody, C3DR antibody, CD21 antibody, Cr-1 antibody, Cr-2 antibody, Cr1 antibody, CR1-L antibody, CR2 antibody, CR1 antibody, complement C3b/C4b receptor 1 (Knops blood group) antibody, complement receptor 2 antibody, complement component (3d/Epstein Barr virus) receptor 2 antibody, complement receptor type 1 antibody, CR1 antibody, Cr2 antibody, CR2 antibody, LOC718120 antibody
- 背景
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The 8C12 antibody recognizes an epitope present on the 190-kDa complement receptor protein, originally designated CR1 (CD35), but not the 145-150-kDa CR2 (CD21) molecule. Unlike the human system, in which these proteins are products of independent genes, both of these mouse receptors are membrane proteins resulting from the alternative splicing of mRNA transcribed from the Cr2 gene. Therefore, an alternative nomenclature has been proposed, designating the proteins Cr2-190 (CD21b) and Cr2-145 (CD21a), respectively. The epitope recognized by 8C12 mAb is only present on CD35/CD21b. Moreover, it has also been proposed that Crry is the true mouse genetic homologue of human CR1 (CD35). In the mouse, CD35 is expressed on the majority of peripheral B cells, on the majority of resident peritoneal macrophages, on peripheral blood granulocytes after treatment with N-formyl-Met-Leu-Phe, and on follicular dendritic cells, but not on thymocytes, T cells, erythrocytes, or platelets. In addition, it has not been detected, at the protein or mRNA level, in the macrophage cell line J774, bone marrow-derived macrophages, or thioglycollate-elicited peritoneal macrophages. The 8C12 mAb has been reported to inhibit rosette formation by C3bbearing sheep erythrocytes, to block the complement-dependent trapping of immune complexes by follicular dendritic cells, and to down-regulate mouse CD35 expression upon in vivo application, inhibiting only some primary antibody responses to immunization. B lymphocytes of Cr2[null] mice display impaired humoral immune responses in vivo. The 8C12 mAb recognizes an epitope on mouse CD35 distinct from the epitope recognized by anti-mouse CD21/CD35 mAb 7G6 (ABIN967384), and it does not block binding by 7G6 mAb to CD35. *Please note that the isotype of 8C12 mAb was originally reported to be Rat IgG2c. Further investigations have demonstrated that the isotype of 8C12 mAb is Rat IgG2a.
Synonyms: CR1, CD21b - 途径
- Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity
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