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CD44 抗体

CD44 适用: 小鼠 FACS, WB, ELISA, IHC (fro), IHC (f), BR 宿主: 大鼠 Monoclonal KM114 unconjugated
产品编号 ABIN967622
发货至: 中国
  • 抗原 See all CD44 抗体
    CD44
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    大鼠
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    单克隆
    标记
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    This CD44 antibody is un-conjugated
    应用范围
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    Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Blocking Reagent (BR)
    品牌
    BD Pharmingen™
    产品特性
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    纯化方法
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    免疫原
    (C57BL/6 x DBA/2)F1 mouse bone marrow-derived stromal cell clone BMS2
    克隆位点
    KM114
    亚型
    IgG1 kappa
    Top Product
    Discover our top product CD44 Primary Antibody
  • 应用备注
    For quantitation of soluble CD44, a sandwich ELISA using purified IM7 antibody for capture and biotinylated KM114 mAb for detection has been described. For IHC, we recommend the use of purified IM7 mAb in our special formulation for immunohistochemistry.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store undiluted at 4° C.
  • Castro, Bono, Simon, Vargas, Rosemblatt: "Spleen-derived stromal cells. Adhesion molecules expression and lymphocyte adhesion to reticular cells." in: European journal of cell biology, Vol. 74, Issue 4, pp. 321-8, (1998) (PubMed).

    Naor, Sionov, Ish-Shalom: "CD44: structure, function, and association with the malignant process." in: Advances in cancer research, Vol. 71, Issue 4, pp. 241-319, (1997) (PubMed).

    Weber, Ashkar, Glimcher, Cantor: "Receptor-ligand interaction between CD44 and osteopontin (Eta-1)." in: Science (New York, N.Y.), Vol. 271, Issue 5248, pp. 509-12, (1996) (PubMed).

    Bendelac: "Mouse NK1+ T cells." in: Current opinion in immunology, Vol. 7, Issue 3, pp. 367-74, (1995) (PubMed).

    Katoh, Zheng, Oritani, Shimozato, Kincade: "Glycosylation of CD44 negatively regulates its recognition of hyaluronan." in: The Journal of experimental medicine, Vol. 182, Issue 2, pp. 419-29, (1995) (PubMed).

    Zheng, Katoh, He, Oritani, Miyake, Lesley, Hyman, Hamik, Parkhouse, Farr, Kincade: "Monoclonal antibodies to CD44 and their influence on hyaluronan recognition." in: The Journal of cell biology, Vol. 130, Issue 2, pp. 485-95, (1995) (PubMed).

    Katoh, McCarthy, Kincade: "Characterization of soluble CD44 in the circulation of mice. Levels are affected by immune activity and tumor growth." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 8, pp. 3440-9, (1994) (PubMed).

    Sprent, Tough: "Lymphocyte life-span and memory." in: Science (New York, N.Y.), Vol. 265, Issue 5177, pp. 1395-400, (1994) (PubMed).

    Lesley, Hyman, Kincade: "CD44 and its interaction with extracellular matrix." in: Advances in immunology, Vol. 54, Issue 4, pp. 271-335, (1993) (PubMed).

    Naujokas, Morin, Anderson, Peterson, Miller: "The chondroitin sulfate form of invariant chain can enhance stimulation of T cell responses through interaction with CD44." in: Cell, Vol. 74, Issue 2, pp. 257-68, (1993) (PubMed).

    Murakami, Miyake, June, Kincade, Hodes: "IL-5 induces a Pgp-1 (CD44) bright B cell subpopulation that is highly enriched in proliferative and Ig secretory activity and binds to hyaluronate." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 11, pp. 3618-27, (1991) (PubMed).

    MacDonald, Budd, Cerottini: "Pgp-1 (Ly 24) as a marker of murine memory T lymphocytes." in: Current topics in microbiology and immunology, Vol. 159, Issue 4, pp. 97-109, (1990) (PubMed).

    Miyake, Medina, Hayashi, Ono, Hamaoka, Kincade: "Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long-term bone marrow cultures." in: The Journal of experimental medicine, Vol. 171, Issue 2, pp. 477-88, (1990) (PubMed).

    Lynch, Ceredig: "Mouse strain variation in Ly-24 (Pgp-1) expression by peripheral T cells and thymocytes: implications for T cell differentiation." in: European journal of immunology, Vol. 19, Issue 2, pp. 223-9, (1989) (PubMed).

  • 抗原
    CD44
    别名
    CD44 (Pgp-1, H-CAM, Ly24) (CD44 产品)
    别名
    CDW44 antibody, CSPG8 antibody, ECMR-III antibody, HCELL antibody, HUTCH-I antibody, IN antibody, LHR antibody, MC56 antibody, MDU2 antibody, MDU3 antibody, MIC4 antibody, Pgp1 antibody, AU023126 antibody, AW121933 antibody, AW146109 antibody, HERMES antibody, Ly-24 antibody, Pgp-1 antibody, CD44A antibody, METAA antibody, RHAMM antibody, XCD44 antibody, cd44 antibody, cdw44 antibody, cspg8 antibody, ecmr-iii antibody, hcell antibody, lhr antibody, mc56 antibody, mdu2 antibody, mdu3 antibody, mic4 antibody, mutch-i antibody, pgp1 antibody, CD44 molecule (Indian blood group) antibody, syndecan 4 antibody, CD44 antigen antibody, CD44 molecule (Indian blood group) S homeolog antibody, CD44 antibody, SDC4 antibody, Cd44 antibody, cd44.S antibody
    背景
    The KM114 antibody reacts with an epitope on both alloantigens and all isoforms of the CD44 glycoprotein (Pgp-1, Ly-24). The KM114 hybridoma was produced at the same time as the published clone KM201, and the mAb recognizes a different epitope from that recognized by mAb IM7. The standard form of CD44, lacking variable exons and referred to as CD44H or CD44S, is widely expressed on hematopoietic and non-hematopoietic cells. CD44 isoforms encoded by variable exons are expressed on epithelial cells, but only at low levels on most leukocytes. Mice with the Ly-24.1 alloantigen (e.g., BALB/c, CBA/J, DBA/1, DBA/2) have relatively large subsets of CD44H+ T lymphocytes, while Ly-24.2 strains (e.g., A, AKR, CBA/N, C3H/He, C57BL, C57BR, C57L, C58, NZB, SJL, SWR, 129) have lower expression of CD44H on T cells. CD44 is a cell adhesion receptor, and its ligand, hyaluronate, is a common component of extracellular matrices. Differential glycosylation of CD44 influences its binding to hyaluronate. Additional ligands include the cell-surface form of CD74 and the cytokine osteopontin (Eta-1).10 Bone marrow- and thymus-derived progenitor cells capable of repopulating the thymus express CD44. In the periphery, the level of CD44 expression increases upon activation of B lymphocytes, CD4+ T cells, and CD8+ T cells, and memory cells can be recognized by their CD44[hi] phenotype. KM114 antibody can be used in ELISA to detect soluble CD44, and it is effective for in vitro blocking of hyaluronate recognition by CD44. It has been reported that KM114 mAb cross-reacts with CD44 of the Chinese hamster, Cricetulus griseus, but not human CD44.
    Synonyms: Pgp-1, H-CAM, Ly-24
    途径
    Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling
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