LCP2 抗体 (pTyr128)
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- 抗原 See all LCP2 抗体
- LCP2 (Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2))
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抗原表位
- pTyr128
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This LCP2 antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 品牌
- BD Pharmingen™
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Phosphorylated Human SLP-76
- 克隆位点
- J141-668-36-58
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product LCP2 Primary Antibody
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- 说明
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Related Products: ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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The SLP-76 family of adapter proteins." in: Seminars in immunology, Vol. 16, Issue 6, pp. 379-93, (2004) (PubMed).
: "Adaptor proteins in lymphocyte activation." in: Current opinion in immunology, Vol. 15, Issue 3, pp. 269-76, (2003) (PubMed).
: "Tyrosines 113, 128, and 145 of SLP-76 are required for optimal augmentation of NFAT promoter activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 9, pp. 3769-73, (1996) (PubMed).
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The SLP-76 family of adapter proteins." in: Seminars in immunology, Vol. 16, Issue 6, pp. 379-93, (2004) (PubMed).
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- 抗原
- LCP2 (Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2))
- 别名
- SLP-76 (LCP2 产品)
- 别名
- AI323664 antibody, BB161688 antibody, SLP-76 antibody, SLP76 antibody, twm antibody, Slp76 antibody, lymphocyte cytosolic protein 2 antibody, Lcp2 antibody, LCP2 antibody
- 背景
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SLP-76 (SH2 domain-containing Leukocyte Protein of 76 kDa) is a tyrosine phosphoprotein that is involved in the T cell receptor (TCR)-mediated intracellular signaling pathway. It may be involved in the signaling pathways of other peripheral blood leukocytes, thymic/splenic cells, and in human T, B, and monocytic cell lines. SLP-76 consists of several motifs that signify its importance in protein-protein interactions involved in intracellular signaling pathways, such as the SH2 domain in the C-terminus, the three amino-terminus 17-amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P), and a proline rich region. SLP-76 has been shown to associate with Gads, Grb2, PLCg1, SLAP-130, and Vav, all of which are part of the signaling cascade in T lymphocytes. An early event in the T cell activation pathway is the phosphorylation, by the Syk-family kinase ZAP-70, of SLP-76 at the three conserved tyrosine motifs, which then mediate interactions with downstream effectors. The phosphorylated tyrosine 128 (Y128) brings the Rho family guanine nucleotide exchange factor Vav1 and the Nck adapter protein, which binds to p21-activated kinase (PAK1) and Wiskott-Aldrich syndrome protein (WASP), into the activation complex.
Vav1, PAK1, and WASP may mediate TCR-stimulated actin cytoskeletal rearrangement. The J141-688.36.58 monoclonal antibody recognizes the phosphorylated Y128 of activated SLP-76. - 分子量
- 76 kDa
- 途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway
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