NFATC1 抗体
(5 references)-
- 抗原 See all NFATC1 抗体
- NFATC1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1))
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This NFATC1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
- 品牌
- BD Pharmingen™
- 交叉反应
- 小鼠
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 克隆位点
- 7A6
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product NFATC1 Primary Antibody
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anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) (AA 20-300) antibody
NFATC1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) (AA 601-716) antibodyNFATC1 适用: 人, 小鼠, 大鼠 ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) (AA 48-406) antibodyNFATC1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) (AA 110-210) antibody (PerCP)NFATC1 适用: 人 WB, IHC 宿主: 兔 Polyclonal PerCP
anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) (AA 110-210) antibody (Atto 594)NFATC1 适用: 人 WB, IHC 宿主: 兔 Polyclonal Atto 594
anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) (AA 110-210) antibody (Atto 488)NFATC1 适用: 人 WB, IHC 宿主: 兔 Polyclonal Atto 488
anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) (AA 110-210) antibody (Atto 390)NFATC1 适用: 人 WB, IHC 宿主: 兔 Polyclonal Atto 390
anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) (C-Term) antibodyNFATC1 适用: 人, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) (AA 428-716) antibodyNFATC1 适用: 人 WB, ELISA, FACS, IF, ICC 宿主: 小鼠 Monoclonal AT1C3 unconjugated
anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) (AA 110-210) antibodyNFATC1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- 7A6 may be used for western blot analysis of NF-ATc1 (1-2 µg/ml). Note that while the predicted M W of NF-ATc is ~100 kDa, the observed M W may range between 100 to 120 kDa due to post-translational modification of NF-ATc. Other applications include immunoprecipitation (2 µg/ml), mobility shift assays (EMSA) and immunofluorescence microscopy of paraformaldehyde-fixed, tissue cultured cells. Jurkat human T cells (ATCC TIB-152) are suggested as a positive control for expression of NF-ATc.
- 说明
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Related Products: ABIN967389, ABIN968537
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "The transcription factor NF-ATc1 regulates lymphocyte proliferation and Th2 cytokine production." in: Immunity, Vol. 8, Issue 1, pp. 115-24, (1998) (PubMed).
: "Redundant expression but selective utilization of nuclear factor of activated T cells family members." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 6, pp. 2735-40, (1997) (PubMed).
: "Rapid shuttling of NF-AT in discrimination of Ca2+ signals and immunosuppression." in: Nature, Vol. 383, Issue 6603, pp. 837-40, (1996) (PubMed).
: "NF-AT components define a family of transcription factors targeted in T-cell activation." in: Nature, Vol. 369, Issue 6480, pp. 497-502, (1994) (PubMed).
: "Contingent genetic regulatory events in T lymphocyte activation." in: Science (New York, N.Y.), Vol. 243, Issue 4889, pp. 355-61, (1989) (PubMed).
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: "The transcription factor NF-ATc1 regulates lymphocyte proliferation and Th2 cytokine production." in: Immunity, Vol. 8, Issue 1, pp. 115-24, (1998) (PubMed).
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- 抗原
- NFATC1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1))
- 别名
- NF-ATc1 (NFATC1 产品)
- 别名
- nf-atc antibody, nfat2 antibody, nfatc antibody, si:ch211-198c22.2 antibody, NFATC1 antibody, 2210017P03Rik antibody, AI449492 antibody, AV076380 antibody, NF-ATc antibody, NFAT2 antibody, NFATc antibody, Nfatcb antibody, NF-ATC antibody, NF-ATc1 antibody, NFAT antibody, NFATmac antibody, nuclear factor of activated T-cells 1 antibody, nuclear factor of activated T-cells 1 L homeolog antibody, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 antibody, nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 1 antibody, NFATC1 antibody, nfatc1.L antibody, nfatc1 antibody, Nfatc1 antibody
- 背景
- The NF-AT family of transcription factors are regulators of early immune response genes in T cells following their activation by CD40L, FasL and nterleukins including IL-2, -3, -4 and -5. NF-AT proteins function in both signal transduction and transcription control. The DNA binding, active NF-AT complex contains a cytoplasmic, Ca2+/calcineurin dependent, cyclosporin sensitive subunit, designated NF-ATc, which is present in the cytoplasm of resting cells. Upon cell activation, NF-ATc is dephosphorylated and thus activated by calcineurin, resulting in rapid translocation of NF-ATc to the nucleus. The active NF-AT complex also contains a nuclear component, NF-ATn. In vitro, NF-AT cooperates with the mitogenic transcription factor AP-1 to induce multiple cytokine genes. NF-ATc is encoded by four genes: NF-ATc1 (originally named NF-ATc), NF-ATc2 (originally named NF-ATp), NF-ATc3 (NF-AT3) and NF- ATc4 (NF-AT4). These proteins are differentially expressed in tissues, suggesting that each may activate distinct sets of genes. NF-ATc1 is expressed in lymphoid organs and is upregulated in activated T cell and thymocytes. NF-ATc1 is required for the development of T cells and has been shown to contribute to the production of IL-2 and the development of Th2 responses. 7A6 recognizes human and mouse NF-ATc1. The antibody recognizes a region between amino acids 197 to 304 of human NF-ATc1, which lies outside the region of homology between NF-ATc1 and NF-ATc2.3 Please note that in the original publication for the antibody (Northrop et al. 1994), the clone name 7A6 is not specifically identified.
- 分子量
- 100-120 kDa
- 途径
- RTK signaling, WNT signaling, Fc-epsilon Receptor Signaling Pathway
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