GFAP 抗体
(3 references)-
- 抗原 See all GFAP 抗体
- GFAP (Glial Fibrillary Acidic Protein (GFAP))
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适用
- 人, 大鼠, 小鼠, Pig, Cow, 小鸡, 兔, 豚鼠, 绵羊, 犬
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This GFAP antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 品牌
- BD Pharmingen™
- 交叉反应
- 人, 小鼠, 牛, 绵羊, 犬, 猪, 兔, 豚鼠, 小鸡, 大鼠
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Cow spinal cord homogenate
- 克隆位点
- 1B4
- 亚型
- IgG2b
- Top Product
- Discover our top product GFAP Primary Antibody
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anti-Glial Fibrillary Acidic Protein (GFAP) (AA 51-150) antibody
GFAP 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), ICC, FACS, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (C-Term) antibodyGFAP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (AA 93-432) antibodyGFAP 适用: 人, 大鼠, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (AA 253-354) antibodyGFAP 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (AA 1-430) antibodyGFAP 适用: 大鼠 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C9 unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (AA 292-432) antibodyGFAP 适用: 人 WB, IHC, IF, ELISA, FACS 宿主: 小鼠 Monoclonal 1C91F1 unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) antibodyGFAP 适用: 人, 大鼠, 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (pSer38) antibodyGFAP 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (C-Term) antibodyVerified GFAP 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) antibodyGFAP 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
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- 应用备注
- Additional GFAP specific antibodies: clone 2E1 (ABIN967501), 4A11 (ABIN967499), and clones 1B4, 4A11, 2E1 combined and available as a cocktail” (ABIN967502). Applications include indirect immunofluorescence of tissue-cultured cells, immunohistochemical staining of formalin-fixed paraffin-embedded brain tissue sections (25 µg/ml), and western blot analysis (1-2 µg/ml). Rat brain is suggested as a positive control
- 说明
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Related Products: ABIN967501, ABIN967499, ABIN967502
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Immunohistochemistry of the glial fibrillary acidic protein: basic and applied considerations." in: Brain pathology (Zurich, Switzerland), Vol. 4, Issue 3, pp. 221-8, (1994) (PubMed).
: "The immunohistochemical application of three anti-GFAP monoclonal antibodies to formalin-fixed, paraffin-embedded, normal and neoplastic brain tissues." in: Journal of neuropathology and experimental neurology, Vol. 45, Issue 6, pp. 692-703, (1986) (PubMed).
: "Monoclonal antibodies reactive with epitopes restricted to glial fibrillary acidic proteins of several species." in: Neurochemical pathology, Vol. 3, Issue 2, pp. 119-38, (1985) (PubMed).
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: "Immunohistochemistry of the glial fibrillary acidic protein: basic and applied considerations." in: Brain pathology (Zurich, Switzerland), Vol. 4, Issue 3, pp. 221-8, (1994) (PubMed).
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- 抗原
- GFAP (Glial Fibrillary Acidic Protein (GFAP))
- 别名
- GFAP (GFAP 产品)
- 别名
- GFAP antibody, AI836096 antibody, cb345 antibody, etID36982.3 antibody, gfapl antibody, wu:fb34h11 antibody, wu:fk42c12 antibody, xx:af506734 antibody, zgc:110485 antibody, glial fibrillary acidic protein antibody, GFAP antibody, LOC100136168 antibody, gfap antibody, Gfap antibody
- 背景
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GFAP (Glial Fibrillary Acid Protein) is the major protein of glial filaments in differentiated astrocytes. We offer a panel ofnmonoclonal antibodies (4A11, 1B4, 2E1) that specifically recognize GFAP. They do not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin, keratin, neurotubules or microfilaments. Bovine spinal cord homogenate was used as immunogen. This antibody has broad species reactivity, recognizing GFAP in brain homogenates from human, mouse, rat, cow, sheep, dog, pig, rabbit, guinea pig and chicken. 1B4 is particularly useful for identifying GFAP in immunohistochemistry of frozen and formalin-fixed, paraffin-embedded brain tissue sections. Additional applications include western blot analysis and indirect immunofluorescence of tissue-cultured cells.
The antibody is routinely tested by immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue samples, which were subjected to pretreatment with citrate or tissue unmasking fluid (see figure). - 分子量
- 50 kDa
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