Cyclin A (CCN-A) 抗体

ABIN967425 产品详细信息, 供应商: Log in to see
抗原
适用

58
34
20
18
2
2
1
1
1
宿主
小鼠
36
31
克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
2
2
1
应用范围
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
58
30
25
20
18
14
10
6
3
2
2
1
1
选项
Supplier
Log in to see
Supplier Product No.
Log in to see
品牌 BD Pharmingen™
免疫原 Human Cyclin A Recombinant Protein
克隆位点 BF683
亚型 IgE
产品特性 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
纯化方法 The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
别名 Cyclin A
背景 Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle. The main cyclin-cdks complexes formed in vertebrate cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small regulatory proteins including p21 and p27Kip1. Specific substrates for cdk-cyclin complexes include nuclear lamins, histones, oncogenes (e.g., c-abl and SV40 large T-Ag), tumor suppressor genes (e.g., retinoblastoma protein, Rb), nucleolin and others. Cyclin A is involved in both S-phase and G2/M transitions of the cell cycle through its association with cdk2 and cdk1, respectively. Cyclin A may also form a complex with the adenovirus oncoprotein E1A which has DNA binding activity. Human cyclin A has been reported to migrate between 54-60 kDa by SDS-PAGE and clone BF683 reportedly does not cross-react with mouse, rat or mink cyclin A.
分子量 54-60 kDa
研究领域 Cardiovascular, Cell Cycle
说明

Related Products: ABIN968586, ABIN967389

限制 仅限研究用
状态 Liquid
浓度 0.5 mg/mL
缓冲液 Aqueous buffered solution containing ≤0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 Store undiluted at 4°C.
厂商提供的图像
Western Blotting (WB) image for anti-Cyclin A (CCN-A) antibody (ABIN967425) Western blot analysis of cyclin A. K-562 cell lysates (Human bone marrow myelogenous ...
背景 Gong, Bhatia, Traganos, Darzynkiewicz: "Expression of cyclins A, D2 and D3 in individual normal mitogen stimulated lymphocytes and in MOLT-4 leukemic cells analyzed by multiparameter flow cytometry." in: Leukemia, Vol. 9, Issue 5, pp. 893-9, 1995 (PubMed).

Gong, Traganos, Darzynkiewicz: "Discrimination of G2 and mitotic cells by flow cytometry based on different expression of cyclins A and B1." in: Experimental cell research, Vol. 220, Issue 1, pp. 226-31, 1995 (PubMed).

Faha, Harlow, Lees: "The adenovirus E1A-associated kinase consists of cyclin E-p33cdk2 and cyclin A-p33cdk2." in: Journal of virology, Vol. 67, Issue 5, pp. 2456-65, 1993 (PubMed).

Sherr: "Mammalian G1 cyclins." in: Cell, Vol. 73, Issue 6, pp. 1059-65, 1993 (PubMed).

Cao, Faha, Dembski, Tsai, Harlow, Dyson: "Independent binding of the retinoblastoma protein and p107 to the transcription factor E2F." in: Nature, Vol. 355, Issue 6356, pp. 176-9, 1992 (PubMed).

Faha, Ewen, Tsai, Livingston, Harlow: "Interaction between human cyclin A and adenovirus E1A-associated p107 protein." in: Science (New York, N.Y.), Vol. 255, Issue 5040, pp. 87-90, 1992 (PubMed).

您还需要查找其他产品吗?