p53 抗体 (Tumor Protein P53) (full length)

Details for Product anti-TP53 Antibody No. ABIN967416, 供应商: Log in to see
抗原
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
抗原表位
full length
173
128
94
84
74
71
70
62
59
55
50
49
48
47
42
38
32
31
29
28
24
22
20
20
17
16
15
15
15
15
15
14
12
11
10
10
9
9
9
8
7
7
7
7
7
7
6
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6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
适用
人, 小鼠, 大鼠
2428
518
425
188
127
94
87
84
35
23
20
18
17
16
15
14
6
4
4
4
4
3
3
3
2
2
1
1
宿主
小鼠
1372
1169
7
3
1
1
1
1
克隆类型 (克隆位点)
单克隆 ()
标记
This p53 antibody is un-conjugated
81
78
65
55
49
48
34
34
26
26
25
25
25
25
25
24
24
24
23
22
22
21
14
14
14
14
14
12
7
5
5
5
5
5
5
5
5
4
3
2
2
2
应用范围
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS), Immunoprecipitation (IP), Western Blotting (WB)
1931
772
751
742
651
637
434
347
315
179
72
41
32
31
24
17
17
15
13
9
8
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
选项
Supplier
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Supplier Product No.
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'独立验证'标志
Lot Number 2335876
Method validated Western Blotting
Positive Control U251-MG cells
Negative Control PC3 cells
Notes Strong bands of the expected size were observed in the positive control sample, and not in the negative control sample.
Primary Antibody
  • Antibody: Tumor Protein P53 (TP53) antibody
  • Catalog number: ABIN967416
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 2335876
Secondary Antibody
Controls
  • U251-MG (positive) and PC3 (negative) cell line extracts were prepared using RIPA buffer (R0278, Sigma Aldrich).
  • Loading control: blots were stripped and re-probed for beta-actin to ensure equal loading of lysates.
实验流程
  • 1. Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25%
  • Beta-mercaptoethanol at 95°C for 5 min prior to loading.
  • 2. 32 µg of boiled extracts were loaded and resolved on a 8-16% SDS-polyacrylamide gel.
  • 3. The Spectra Multicolor Broad Range molecular mass marker (26634 Thermo Scientific) was used as a
  • standard.
  • 4. Proteins were then transferred onto PVDF membrane by tank transfer and protein transfer was
  • confirmed with Ponceau S staining.
  • 5. The immunoblot membrane was blocked in PBS containing 3% (W/V) non-fat dry milk at room
  • temperature for 1 h.
  • 6. The membrane was rinsed with PBS containing 0.05% Tween-20 once.
  • 7. The membrane was immersed with the protein side up in the antibody solution in PBS containing 1%
  • (W/V) non-fat dry milk and incubated for 2 h at room temperature (~26°C).
  • 8. The membrane was rinsed in PBS containing 0.05% Tween-20 thrice for 10 min each.
  • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution in PBS containing
  • 1% (W/V) non-fat dry milk and incubated for 1 h at room temperature (~26°C) with gentle agitation.
  • 10. The membrane was rinsed thrice PBS containing 0.05% Tween-20 thrice for 10 min each.
  • 11. The membrane was washed in PBS twice for 30 sec each.
  • 12. Signals were detected with Pierce ECL Western Blotting Substrate (32109, Thermo Scientific). The
  • blot was scanned for 300 sec.
  • 13. The membrane was rinsed three times with PBS containing 0.05% Tween-20.4. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10
  • min each.
  • 15. The membrane was washed in PBS containing 0.05% Tween-20 times for 10 min each.
  • 16. Repeated Steps 5-12 with the loading control antibody (beta-actin) and its matching secondary
  • antibody.
Experimental Notes None
Validation Images
Western Blotting validation image for anti-Tumor Protein P53 (TP53) (full length) antibody (ABIN967416) Figure 1: Western blot analysis of U251-MG and PC3 cell line extracts using Tumor Pro...
品牌 BD Pharmingen™
免疫原 Recombinant full-length human p53
克隆位点 G59-12
亚型 IgG1
交叉反应 小鼠, 大鼠
产品特性 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
纯化方法 The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
别名 p53 (TP53 Antibody 摘要)
背景 P53 is a 53 kD nuclear phosphoprotein that acts as a tumor suppressor protein, and is involved in inhibiting cell proliferation when DNA damage occurs. The gene for p53 is the most commonly mutated gene yet identified in human cancers. Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is overexpressed in a variety of transformed cells and the wildtype p53 forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus and E6 from human papilloma virus. Wildtype p53 plays a role as a checkpoint protein for DNA damage during the S-phase of the cell cycle. p53 migrates at a reduced molecular weight of 53 kDa. Clone G59-12 recognizes mutant and wild type human, rat and mouse p53 tumor suppressor protein. Recombinant full-length human p53 was used as immunogen.
分子量 53 kDa
研究领域 Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis
途径 p53 Pathway, MAPK Pathway, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy
应用备注 Clone G59-12 conjugated to R-Phycoerythrin (PE) is suggested for flow cytometric analysis of p53. Positive control cell lines include SKBR-3 human breast carcinoma cells (ATCC HTB-30) and A431 human vulval carcinoma cells (ATCC CRL-1555). Jurkat T cells (ATCC TIB-152) or MCF-7 human breast carcinoma cells (ATCC HTB-22) are suggested as negative controls. Positive immunostaining is seen in a high proportion of breast and colon carcinomas. p53 staining is not typically detected in normal skin, brain, kidney, lung, stomach, or breast tissue.
限制 仅限研究用
状态 Liquid
浓度 0.5 mg/mL
缓冲液 Aqueous buffered solution containing ≤0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 Store undiluted at 4°C.
厂商提供的图像
Western Blotting (WB) image for anti-Tumor Protein P53 (TP53) (full length) antibody (ABIN967416) Western blot analysis of p53. A SV-40 transformed rat granulosa cell lysate was probe...
 image for anti-Tumor Protein P53 (TP53) (full length) antibody (ABIN967416) anti-Tumor Protein P53 (TP53) (full length) antibody (Image 2)
有引用在: Van Meir, Roemer, Diserens, Kikuchi, Rempel, Haas, Huang, Friedmann, de Tribolet, Cavenee: "Single cell monitoring of growth arrest and morphological changes induced by transfer of wild-type p53 alleles to glioblastoma cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 4, pp. 1008-12, 1995 (PubMed).

Jacquemier, Molès, Penault-Llorca, Adélaide, Torrente, Viens, Birnbaum, Theillet: "p53 immunohistochemical analysis in breast cancer with four monoclonal antibodies: comparison of staining and PCR-SSCP results." in: British journal of cancer, Vol. 69, Issue 5, pp. 846-52, 1994 (PubMed).

Mørkve, Halvorsen, Stangeland, Gulsvik, Laerum: "Quantitation of biological tumor markers (p53, c-myc, Ki-67 and DNA ploidy) by multiparameter flow cytometry in non-small-cell lung cancer." in: International journal of cancer. Journal international du cancer, Vol. 52, Issue 6, pp. 851-5, 1993 (PubMed).

van den Berg, Baas, Polak, Offerhaus: "Detection of p53 overexpression in routinely paraffin-embedded tissue of human carcinomas using a novel target unmasking fluid." in: The American journal of pathology, Vol. 142, Issue 2, pp. 381-5, 1993 (PubMed).

Yeargin, Cheng, Yu, Gjerset, Bogart, Haas: "P53 mutation in acute T cell lymphoblastic leukemia is of somatic origin and is stable during establishment of T cell acute lymphoblastic leukemia cell lines." in: The Journal of clinical investigation, Vol. 91, Issue 5, pp. 2111-7, 1993 (PubMed).

Cheng, Yee, Yeargin, Friedmann, Haas: "Suppression of acute lymphoblastic leukemia by the human wild-type p53 gene." in: Cancer research, Vol. 52, Issue 1, pp. 222-6, 1992 (PubMed).

Yeargin, Cheng, Haas: "Role of the p53 tumor suppressor gene in the pathogenesis and in the suppression of acute lymphoblastic T-cell leukemia." in: Leukemia, Vol. 6 Suppl 3, pp. 85S-91S, 1992 (PubMed).

Vogelstein: "Cancer. A deadly inheritance." in: Nature, Vol. 348, Issue 6303, pp. 681-2, 1991 (PubMed).

背景 Vojt?sek, Bártek, Midgley, Lane: "An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53." in: Journal of immunological methods, Vol. 151, Issue 1-2, pp. 237-44, 1992 (PubMed).

Gjerset, Arya, Volkman, Haas: "Association of induction of a fully tumorigenic phenotype in murine radiation-induced T-lymphoma cells with loss of differentiation antigens, gain of CD44, and alterations in p53 protein levels." in: Molecular carcinogenesis, Vol. 5, Issue 3, pp. 190-8, 1992 (PubMed).

Stein, Stoica, Tilley, Burghardt: "Rat ovarian granulosa cell culture: a model system for the study of cell-cell communication during multistep transformation." in: Cancer research, Vol. 51, Issue 2, pp. 696-706, 1991 (PubMed).

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