p53 抗体 (Tumor Protein P53)

Details for Product anti-TP53 Antibody No. ABIN967409, 供应商: Log in to see
抗原
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Alternatives
anti-人 p53 抗体 for Immunofluorescence (fixed cells)
适用
Hamster, 人, 猴, 小鼠, 大鼠
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513
418
185
127
94
84
82
34
23
20
18
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14
6
4
4
4
4
3
3
3
2
2
1
1
宿主
小鼠
1322
1112
7
3
3
1
1
1
克隆类型 (克隆位点)
单克隆 ()
标记
This p53 antibody is un-conjugated
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76
63
53
45
44
30
30
25
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14
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7
5
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5
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2
2
应用范围
Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
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746
719
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423
340
300
179
72
41
32
31
18
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15
13
9
9
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Supplier
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品牌 BD Pharmingen™
免疫原 SV40-transformed mouse cell line BALB/c 3T3 clone 4 (B4)
克隆位点 PAb 122
亚型 IgG2b
交叉反应 人, 大鼠, Hamster, 猴
产品特性 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
纯化方法 The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
别名 p53 (TP53 Antibody 摘要)
背景 The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers.1 Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is overexpressed in a variety of transformed cells and it forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus, and E6 from human papilloma virus. Wild type p53 plays a role as a checkpoint protein for DNA damage during the G1/S-phase of the cell cycle. However, it is still unclear, whether point mutated forms of p53 are simple null mutants and/or dominant negatively acting proteins. p53 migrates at a reduced molecular weight of 53 kDa. PAb 122 recognizes mammalian p53, including mouse, rat, hamster, monkey and human p53.2 Cells from the SV40-transformed mouse cell line BALB/c 3T3 clone 4 (B4) were used as immunogen. PAb 122 was originally evaluated by ELISA, immunoprecipitation, radioimmunoassays and indirect immunofluorescence of cultured cells.
分子量 53 kDa
研究领域 Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis
途径 p53 Pathway, MAPK Pathway, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy
应用备注 Applications include immunoprecipitation (1-2 µg/1x10^6 cells), western blot analysis (2 µg/ml), immunofluorescence microscopy of cultured cells and flow cytometry. COS-7 SV40 transformed monkey kidney cells (ATCC CRL-1651) or another SV40-transformed cell line are suggested as positive controls for detecting p53. COS-7 cell lysate is optimized for a western blot control and comes ready to load on an SDS-PAGE gel.
限制 仅限研究用
状态 Liquid
浓度 0.5 mg/mL
缓冲液 Aqueous buffered solution containing ≤0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 Store undiluted at 4°C.
厂商提供的图像
Western Blotting (WB) image for anti-p53 抗体 (Tumor Protein P53) (ABIN967409) Western blot analysis of p53. Lysate from COS-7 SV40 transformed monkey kidney cells ...
 image for anti-p53 抗体 (Tumor Protein P53) (ABIN967409) anti-Tumor Protein P53 (TP53) antibody (Image 2)
Western Blotting (WB) image for anti-p53 抗体 (Tumor Protein P53) (ABIN967409) anti-Tumor Protein P53 (TP53) antibody (Image 3)
有引用在: Legros, Lacabanne, dAgay, Larsen, Pla, Soussi: "Production of human p53 specific monoclonal antibodies and their use in immunohistochemical studies of tumor cells." in: Bulletin du cancer, Vol. 80, Issue 2, pp. 102-10, 1994 (PubMed).

Vogelstein: "Cancer. A deadly inheritance." in: Nature, Vol. 348, Issue 6303, pp. 681-2, 1991 (PubMed).

Diller, Kassel, Nelson, Gryka, Litwak, Gebhardt, Bressac, Ozturk, Baker, Vogelstein: "p53 functions as a cell cycle control protein in osteosarcomas." in: Molecular and cellular biology, Vol. 10, Issue 11, pp. 5772-81, 1990 (PubMed).

Yewdell, Gannon, Lane: "Monoclonal antibody analysis of p53 expression in normal and transformed cells." in: Journal of virology, Vol. 59, Issue 2, pp. 444-52, 1986 (PubMed).

背景 Gurney, Harrison, Fenno: "Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens." in: Journal of virology, Vol. 34, Issue 3, pp. 752-63, 1980 (PubMed).