(5 references)-
- 抗原 See all WNT16 抗体
- WNT16 (Wingless-Type MMTV Integration Site Family, Member 16 (WNT16))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This WNT16 antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 品牌
- BD Pharmingen™
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human WNT16 Recombinant Protein
- 克隆位点
- F4-1582
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product WNT16 Primary Antibody
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anti-Wingless-Type MMTV Integration Site Family, Member 16 (WNT16) (AA 30-365) antibody
WNT16 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 16 (WNT16) (C-Term) antibodyWNT16 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 16 (WNT16) (AA 236-265), (C-Term) antibodyWNT16 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB33027 unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 16 (WNT16) (AA 220-265) antibodyWNT16 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 16 (WNT16) (Internal Region) antibodyWNT16 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 16 (WNT16) (Internal Region) antibodyWNT16 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 16 (WNT16) (AA 270-319) antibodyWNT16 适用: 人, 兔, Cow, 豚鼠, 马, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 16 (WNT16) (AA 252-301) antibodyWNT16 适用: 人, 小鼠, 大鼠, 兔, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 16 (WNT16) (Internal Region) antibodyWNT16 适用: 人, Gorilla IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 16 (WNT16) (Internal Region) antibodyWNT16 适用: 人, 小鼠, 大鼠, Hamster IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Applications include western blot analysis (0.063 - 0.25 µg/ml). 697 cell lysate [50 µg (1 µg/µl)] is provided as a positive control (51-9000020).
- 说明
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Related Products: ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the undiluted antibody at 4°C. Store 697 cell lysate at -20°C.
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: "The Hedgehog and Wnt signalling pathways in cancer." in: Nature, Vol. 411, Issue 6835, pp. 349-54, (2001) (PubMed).
: "Wnt signaling and cancer." in: Genes & development, Vol. 14, Issue 15, pp. 1837-51, (2000) (PubMed).
: "Oncogenic homeodomain transcription factor E2A-Pbx1 activates a novel WNT gene in pre-B acute lymphoblastoid leukemia." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 20, pp. 11464-9, (1999) (PubMed).
: "Mechanisms of Wnt signaling in development." in: Annual review of cell and developmental biology, Vol. 14, pp. 59-88, (1999) (PubMed).
: "Wnt signaling: a common theme in animal development." in: Genes & development, Vol. 11, Issue 24, pp. 3286-305, (1998) (PubMed).
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: "The Hedgehog and Wnt signalling pathways in cancer." in: Nature, Vol. 411, Issue 6835, pp. 349-54, (2001) (PubMed).
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- 抗原
- WNT16 (Wingless-Type MMTV Integration Site Family, Member 16 (WNT16))
- 别名
- Wnt16 (WNT16 产品)
- 别名
- zgc:77293 antibody, E130309I19Rik antibody, Wnt family member 16 antibody, wingless-type MMTV integration site family, member 16 antibody, WNT16 antibody, wnt16 antibody, Wnt16 antibody
- 背景
- The Wnt gene family comprises a large group of signaling proteins involved in developmental pathways in vertebrates, Drosophilia, C. elegans and other organisms. The Wnt genes were first discovered in mouse (called int-1) and later in Drosophilia (called wingless, or wg) where the signaling pathways were unravelled. The Wnt proteins are involved in various developmental processes including embryonic induction, generation of cell polarity and the specification of cell fate. The vertebrate Wnt glycoproteins number at least 16 members and initiate signaling by being secreted and a subset of these glycoproteins bind to a class of receptors, called Frizzled, of which 11 have been identified. Stimulation of the Wnt pathway causes the phosphorylation of Dishevelled, which inhibits glycogen synthase kinase -3beta (GSK3beta) and allows beta-catenin to accumulate in the cytosol. beta-catenin then translocates to the nucleus to form a complex with Tcf/LEF family of transcription factors to activate transcription. In unstimulated cells, beta-catenin forms a complex with the proteins Axin, adenomatous polyposis coli (APC), (GSK3ß) and is unstable. Studies have shown that some members of this pathway become mutated in human cancers, such as colon carcinoma and melanoma. Futhermore, studies on the WNT-16 gene, have shown that it is activated by the E2A-Pbx fusion product in acute lymphoblastoid leukemia. Wnt16 has a predicted molecular weight of 40 kDa (SWISS-PROT:Q9UBV4).
- 分子量
- 40 kDa
- 途径
- WNT signaling
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