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- 抗原 See all His Tag 抗体
- His Tag
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This His Tag antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP)
- 品牌
- BD Pharmingen™
- 序列
- HHHHHH
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 克隆位点
- F24-796
- 亚型
- IgG2b
- Top Product
- Discover our top product His Tag Primary Antibody
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- 应用备注
- An E. coli produced recombinant 6xHis control lysate is provided as a positive control. Store lysates at -20°C.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues." in: Journal of chromatography, Vol. 411, pp. 177-84, (1988) (PubMed).
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New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues." in: Journal of chromatography, Vol. 411, pp. 177-84, (1988) (PubMed).
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- 抗原
- His Tag
- 别名
- 6xHis (His Tag 产品)
- 物质类
- Tag
- 背景
- The engineering of a short epitope tag to a recombinant protein provides a very convenient method for detecting and isolating the protein of interest. For example, the placement of a series of 6 consecutive histidine residues that are incorporated at either end of the recombinant fusion protein facilitates the purification of that protein. Purification can be easily accomplished by metal chelate affinity chromatography using a metal resin column. The 6xHis monoclonal antibody can be used to detect the epitope-tagged protein of interest.
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