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IKBKB 抗体 (AA 1-666)

Name: IKBKB 抗体 (AA 1-666)
SKU: ABIN967355
Availability: OutOfStock

IKBKB 适用: 人 WB, IP 宿主: 小鼠 Monoclonal F18-1875 unconjugated

1 image

(10 references)

产品编号 ABIN967355

发货至: 中国

Datasheet as PDF

抗原 See all IKBKB 抗体

IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))
抗原表位
29

29

16

16

16

15

9

9

8

8

8

8

8

8

8

8

7

7

7

7

7

5

4

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1-666
适用
281

113

72

8

7

4

4

3

3

3

1

1
人
宿主
287

19

1
小鼠
Compatible Secondaries
克隆类型
284

23
单克隆
标记
130

24

24

23

18

16

7

7

7

7

7

7

6

6

6

6

6
This IKBKB antibody is un-conjugated
应用范围
160

115

107

80

80

30

29

25

21

20

14

10

4

3

1

1
Western Blotting (WB), Immunoprecipitation (IP)

品牌

BD Pharmingen™

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Human IKKbeta recombinant protein aa. 1-666

克隆位点

F18-1875

亚型

IgG1

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anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) antibody
IKBKB 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Monoclonal 3E1 unconjugated Recombinant Antibody

anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) (AA 3-120) antibody
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应用备注

Applications include western blot analysis (0.06 - 0.25 µg/ml). Other applications not routinely tested include immunoprecipitation (4 µg/sample). Store the antibody at -20°C. HeLa control lysate [50 µg (1 µg/µl)] is provided as a positive control (store lysate at -20°C). HeLa control lysate (ABIN968535) is sold separately as a ready-to-use western blot control.

说明

Related Products: ABIN967389, ABIN968535

限制

仅限研究用
状态

Liquid

浓度

0.25 mg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20°C.
Israël: "The IKK complex: an integrator of all signals that activate NF-kappaB?" in: Trends in cell biology, Vol. 10, Issue 4, pp. 129-33, (2000) (PubMed).

Chu, Ostertag, Li, Chang, Chen, Hu, Williams, Perrault, Karin: "JNK2 and IKKbeta are required for activating the innate response to viral infection." in: Immunity, Vol. 11, Issue 6, pp. 721-31, (2000) (PubMed).

Li, Van Antwerp, Mercurio, Lee, Verma: "Severe liver degeneration in mice lacking the IkappaB kinase 2 gene." in: Science (New York, N.Y.), Vol. 284, Issue 5412, pp. 321-5, (1999) (PubMed).

Li, Chu, Hu, Delhase, Deerinck, Ellisman, Johnson, Karin: "The IKKbeta subunit of IkappaB kinase (IKK) is essential for nuclear factor kappaB activation and prevention of apoptosis." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1839-45, (1999) (PubMed).

Tanaka, Fuentes, Yamaguchi, Durnin, Dalrymple, Hardy, Goeddel: "Embryonic lethality, liver degeneration, and impaired NF-kappa B activation in IKK-beta-deficient mice." in: Immunity, Vol. 10, Issue 4, pp. 421-9, (1999) (PubMed).

Ling, Cao, Goeddel: "NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3792-7, (1998) (PubMed).

Nakano, Shindo, Sakon, Nishinaka, Mihara, Yagita, Okumura: "Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3537-42, (1998) (PubMed).

Rothwarf, Zandi, Natoli, Karin: "IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex." in: Nature, Vol. 395, Issue 6699, pp. 297-300, (1998) (PubMed).

Lee, Hagler, Chen, Maniatis: "Activation of the IkappaB alpha kinase complex by MEKK1, a kinase of the JNK pathway." in: Cell, Vol. 88, Issue 2, pp. 213-22, (1997) (PubMed).

Malinin, Boldin, Kovalenko, Wallach: "MAP3K-related kinase involved in NF-kappaB induction by TNF, CD95 and IL-1." in: Nature, Vol. 385, Issue 6616, pp. 540-4, (1997) (PubMed).
抗原

IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))

别名

IKK beta (IKBKB 产品)

别名

ikk-beta antibody, ikk2 antibody, ikkb antibody, ikkbeta antibody, IKBKB antibody, IKKB antibody, CG4201 antibody, DIK antibody, DLAK antibody, DmIKK antibody, DmIKKBeta antibody, DmIKKb antibody, DmIKKbeta antibody, DmIkkbeta antibody, Dmel\\CG4201 antibody, Dmikkb antibody, Dmikkbeta antibody, IK antibody, IKK antibody, IKK-beta antibody, IKKb antibody, IKKbeta antibody, IRD-5 antibody, IRD5 antibody, Ik antibody, Ikkb antibody, Ikkbeta antibody, Ird-5 antibody, Ird5 antibody, LRK antibody, Lak antibody, anon-89Bd antibody, dIKK antibody, dIKK-beta antibody, dIKKbeta antibody, dLak antibody, dmIKKbeta antibody, ikk antibody, ird antibody, ird5/DmIkk[beta] antibody, IKK2 antibody, NFKBIKB antibody, AIM-1 antibody, AI132552 antibody, IKK-2 antibody, IKK[b] antibody, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta L homeolog antibody, inhibitor of nuclear factor kappa B kinase subunit beta antibody, I-kappaB kinase beta antibody, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta antibody, inhibitor of kappaB kinase beta antibody, ikbkb.L antibody, IKBKB antibody, ikbkb antibody, IKKbeta antibody, Ikbkb antibody

背景

Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. The nuclear transcription factor kappa-B, NF-kappaB, is controlled by interaction with an inhibitory subunit, I-kappaB, which restricts NF-kappaB to the cytoplasm. Following stimulation by various cytokines or other stimuli, I-kappaB becomes degraded and NF-kappaB is released to the nucleus. The release of I-kappaB from NF-kappaB is thought to be a critical point in the activation of NF-kappaB signal pathways. A group of proteins form an NF-kappaB regulatory complex, or signalsome. Two members of this complex are a pair of closely related serine/threonine kinases, IKKalpha and IKKbeta (also called IKK-1 and IKK-2), which phosphorylate critical residues of I-kappaB, thus targeting it for subsequent degradation. The IKK complex contains similar amounts of IKKalpha, IKKbeta, as well as two other polypeptides, which are differentially processed forms of a third subunit, IKKgamma. IKKalpha and IKKbeta become activated following phosphorylation by upstream kinases, including NF-kappaB-inducing kinase (NIK) and MEKK1. IKKgamma interacts preferentially with IKKbeta and is required for the activation of the IKK complex. Experiments done on mice lacking IKKbeta demonstrate that these mice died at mid-gestation from uncontrolled liver apoptosis. Furthermore, when cells from IKKbeta-deficient m ice were treated with tumor necrosis factor alpha or interleukin 1, neither IKK nor NF-kappaB were activated. Thus IKKbeta, but not IKKalpha, plays a pivotal role in the induction of NF-kappaB and activation of IKK. IKKbeta migrates at ~87 kDa in SDS/PAGE. The antibody recognizes human IKKbeta. A recombinant protein corresponding to amino acids 1-666 of human IKKbeta was used as the immunogen.

途径

NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins