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Caspase 8 抗体

CASP8 适用: 人 WB 宿主: 小鼠 Monoclonal 4-1 unconjugated
产品编号 ABIN967336
发货至: 中国
  • 抗原 See all Caspase 8 (CASP8) 抗体
    Caspase 8 (CASP8)
    适用
    • 136
    • 70
    • 63
    • 23
    • 16
    • 16
    • 10
    • 1
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    • 1
    宿主
    • 130
    • 24
    • 5
    • 2
    小鼠
    克隆类型
    • 127
    • 33
    单克隆
    标记
    • 96
    • 16
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    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
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    • 2
    • 2
    • 2
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    • 2
    This Caspase 8 antibody is un-conjugated
    应用范围
    • 142
    • 50
    • 44
    • 38
    • 27
    • 26
    • 25
    • 21
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    • 18
    • 8
    • 4
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    Western Blotting (WB)
    品牌
    BD Pharmingen™
    产品特性
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    纯化方法
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    免疫原
    Human caspase-8 recombinant protein
    克隆位点
    4-1
    亚型
    IgG1 kappa
    Top Product
    Discover our top product CASP8 Primary Antibody
  • 应用备注
    Applications include western blot analysis (1 - 2 µg/ml). Jurkat T cells (ATCC CRL-1573) are suggested as positive controls. Antibodies-online offers several caspase-8 antibodies. A Jurkat model cell system was used to evaluate these antibodies, these results are summarized in the following table. However, actual bands observed could vary according to the cell model system or treatment used.
    说明

    Related Products: ABIN967389, ABIN968537

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store undiluted at 4°C.
  • Boesen-de Cock, Tepper, de Vries, van Blitterswijk, Borst: "Common regulation of apoptosis signaling induced by CD95 and the DNA-damaging stimuli etoposide and gamma-radiation downstream from caspase-8 activation." in: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 14255-61, (1999) (PubMed).

    Thome, Martinon, Hofmann, Rubio, Steiner, Schneider, Mattmann, Tschopp: "Equine herpesvirus-2 E10 gene product, but not its cellular homologue, activates NF-kappaB transcription factor and c-Jun N-terminal kinase." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 9962-8, (1999) (PubMed).

    Cock, Tepper, de Vries, van Blitterswijk, Borst: "CD95 (Fas/APO-1) induces ceramide formation and apoptosis in the absence of a functional acid sphingomyelinase." in: The Journal of biological chemistry, Vol. 273, Issue 13, pp. 7560-5, (1998) (PubMed).

  • 抗原
    Caspase 8 (CASP8)
    别名
    Caspase-8 (CASP8 产品)
    别名
    casp8-A antibody, xCaspase-8 antibody, casp8 antibody, CASP8 antibody, CASP-8 antibody, FLICE antibody, MACH antibody, Mch5 antibody, ALPS2B antibody, CAP4 antibody, Casp-8 antibody, MCH5 antibody, caspase-8 antibody, zgc:92075 antibody, caspase 8 L homeolog antibody, death related ced-3/Nedd2-like protein antibody, caspase 8 antibody, caspase-8 antibody, caspase-8-like antibody, caspase 8, apoptosis-related cysteine peptidase antibody, casp8.L antibody, LOC661095 antibody, CASP8 antibody, LOC5563818 antibody, casp8 antibody, LOC100222284 antibody, Casp8 antibody
    背景
    Caspase-8 (FLICE/MACH-1) is a 55 kDa cytosolic protein with homology to the CD95/Fas-associated signal transducer, FADD/MORT-1, as well as to other caspase (ICE/Ced-3) cysteine proteases. The N-terminal region of caspase-8 contains an amino acid sequence, termed the death domain, that facilitates caspase-8-FADD direct interaction. FADD therefore acts as an adapter molecule, allowing caspase-8 to become recruited to the cytoplasmic region of Fas following receptor activation. Viral proteins (v-FLIPS) which inhibit recruitment and activation of caspase-8 have been isolated. Caspase-8 is produced as a proenzyme (55/50 kDa doublet) which upon receptor aggregation is proteolytically cleaved into smaller subunits of 40/36 (doublet), and 23 kDa. Overexpression of caspase-8 is sufficient to induce apoptosis in certain cell lines (e.g., MCF-7) and this phenotype is blocked by overexpression of the caspase-3 protease inhibitor, CrmA. The antibody recognizes both the proform of human caspase-8 (55/50 kDa doublet) as well as the cleaved form (40/36 kDa doublet) on SDS/PAGE. Full-length recombinant human caspase-8 protein was used as immunogen.
    Synonyms: FLICE, MACH-1, Mch5
    分子量
    55/50 kDa
    途径
    Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades
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