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Bcl-2 抗体 (AA 41-54)

Name: Bcl-2 抗体 (AA 41-54)
SKU: ABIN967321
Availability: OutOfStock

BCL2 适用: 人 WB, FACS, IHC (f), IHC (fro) 宿主: 小鼠 Monoclonal unconjugated

Western blot analysis of Bcl-2 (Second Panel).

Formalin-fixed, paraffin-embedded tissue sections stained for Bcl-2 expression using clone Bcl-2/100 and the SA-HRP method.

IHC (p)

FACS

3 images

(7 references)

产品编号 ABIN967321

发货至: 中国

Datasheet as PDF

抗原 See all Bcl-2 (BCL2) 抗体

Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
抗原表位
51

34

23

22

21

18

17

16

16

16

15

14

13

10

8

8

8

8

7

7

7

6

6

6

6

6

6

5

4

4

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
AA 41-54
适用
444

184

179

38

34

22

22

17

17

16

10

5

5

1

1

1
人
宿主
357

111

6

2

1
小鼠
Compatible Secondaries
克隆类型
354

122
单克隆
标记
210

34

34

25

24

15

11

11

11

11

11

11

9

9

9

9

9

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
This Bcl-2 antibody is un-conjugated
应用范围
390

192

168

121

107

100

92

68

64

33

26

23

18

8

8

8

5

4

2

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro))

品牌

BD Pharmingen™

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Human Bcl-2 synthetic peptide aa. 41-54

亚型

IgG1

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anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 2-211) antibody
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anti-B-Cell CLL/lymphoma 2 (BCL2) antibody
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anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 1-100) antibody
BCL2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-B-Cell CLL/lymphoma 2 (BCL2) antibody
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anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 2-211) antibody
BCL2 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal D1 unconjugated

anti-B-Cell CLL/lymphoma 2 (BCL2) antibody
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anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody
BCL2 适用: 人 WB, IHC, FACS, IF, StM, ISt 宿主: 小鼠 Monoclonal 100-D5 unconjugated

应用备注

Western Blot: Jurkat control lysate [50 µg (1 µg/µl)] is provided as a western blot positive control (Store lysate at -20° C). Additional control lysate (ABIN968537) is sold separately.
IHC: For paraffin-embedded sections, tissues should be fixed in either Bouin's fixative or formalin. For immunofluorescent staining and flow cytometry, our directly conjugated formats are recommended.

说明

Related Products: ABIN968537

限制

仅限研究用
状态

Liquid

浓度

0.25 mg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20°C.
Krajewski, Tanaka, Takayama, Schibler, Fenton, Reed: "Investigation of the subcellular distribution of the bcl-2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes." in: Cancer research, Vol. 53, Issue 19, pp. 4701-14, (1993) (PubMed).

Pezzella, Turley, Kuzu, Tungekar, Dunnill, Pierce, Harris, Gatter, Mason: "bcl-2 protein in non-small-cell lung carcinoma." in: The New England journal of medicine, Vol. 329, Issue 10, pp. 690-4, (1993) (PubMed).

Tanaka, Saito, Reed: "Structure-function analysis of the Bcl-2 oncoprotein. Addition of a heterologous transmembrane domain to portions of the Bcl-2 beta protein restores function as a regulator of cell survival." in: The Journal of biological chemistry, Vol. 268, Issue 15, pp. 10920-6, (1993) (PubMed).

Pezzella, Jones, Ralfkiaer, Ersbøll, Gatter, Mason: "Evaluation of bcl-2 protein expression and 14;18 translocation as prognostic markers in follicular lymphoma." in: British journal of cancer, Vol. 65, Issue 1, pp. 87-9, (1992) (PubMed).

Reed, Meister, Tanaka, Cuddy, Yum, Geyer, Pleasure: "Differential expression of bcl2 protooncogene in neuroblastoma and other human tumor cell lines of neural origin." in: Cancer research, Vol. 51, Issue 24, pp. 6529-38, (1992) (PubMed).

Williams: "Programmed cell death: apoptosis and oncogenesis." in: Cell, Vol. 65, Issue 7, pp. 1097-8, (1991) (PubMed).

Pezzella, Tse, Cordell, Pulford, Gatter, Mason: "Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation." in: The American journal of pathology, Vol. 137, Issue 2, pp. 225-32, (1990) (PubMed).
抗原

Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

别名

Bcl-2 (BCL2 产品)

别名

Bcl-2 antibody, PPP1R50 antibody, bcl-2 antibody, AW986256 antibody, C430015F12Rik antibody, D630044D05Rik antibody, D830018M01Rik antibody, BCL-2 antibody, Bcl2 antibody, BCL2, apoptosis regulator antibody, B cell leukemia/lymphoma 2 antibody, B-cell CLL/lymphoma 2a antibody, B-cell CLL/lymphoma 2 protein antibody, BCL2 antibody, Bcl2 antibody, bcl2a antibody, BCL-2 antibody

背景

Bcl-2 is considered to be novel among proto-oncogenes because it blocks apoptosis (programmed cell death) in many cell types. Apoptosis is an active form of cellular suicide that typically requires new RNA and protein synthesis and is associated with distinct morphological changes including cell shrinkage, cytoplasm membrane blebbing, nuclear fragmentation and DNA degradation. Because Bcl-2 blocks apoptosis it may contribute to tumorigenisis by prolonging cell survival, rather than by accelerating the rate of cell proliferation. Human Bcl-2 protein migrates at a molecular weight of ~26 kDa by SDS-PAGE. Bcl-2/100 recognizes a 26 kDa band representing human Bcl-2. Additional minor bands at 27-31 kDa and 18-21 kDa may also be visualized. The 27-31 kDa upper band may represent a larger isoform, whereas the 18-21 kDa lower band may be an internal translation or proteolytic product, therefore, a synthetic peptide corresponding to amino acids 41-54 (GAAPAPGIFSSQPG) of human Bcl-2 was used as immunogen. This peptide sequence is not conserved between human and mouse. Bcl-2/100 does not cross-react with mouse Bcl-2. For detection of mouse Bcl-2 refer to clone 3F11 (ABIN967441), polyclonal rabbit anti-rat/mouse Bcl-2 antiserum, and polyclonal rabbit anti-mouse Bcl-2 antiserum.

途径

MAPK Pathway, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling