BCL2 抗体 (B-Cell CLL/lymphoma 2)

Details for Product anti-BCL2 Antibody No. ABIN967311, 供应商: Log in to see
抗原
  • Bcl-2
  • PPP1R50
  • bcl-2
  • AW986256
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • BCL-2
  • Bcl2
  • B-cell CLL/lymphoma 2
  • B cell leukemia/lymphoma 2
  • B-cell leukemia/lymphoma 2
  • B-cell leukemia/lymphoma 2-like
  • B-cell CLL/lymphoma 2 protein
  • BCL2
  • Bcl2
  • LOC100224888
  • bcl2
  • LOC100359045
  • BCL-2
适用

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宿主
仓鼠
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克隆类型 (克隆位点)
单克隆 ()
标记
This BCL2 antibody is un-conjugated
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应用范围
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
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选项
Supplier
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Supplier Product No.
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品牌 BD Pharmingen™
免疫原 Human Bcl-2 Recombinant Protein
克隆位点 6C8
亚型 IgG
产品特性 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
纯化方法 The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
别名 Bcl-2 (BCL2 Antibody 摘要)
背景 Bcl-2 is considered to be novel among proto-oncogenes because it blocks apoptosis (programmed cell death) in many cell types. Apoptosis is an active form of cellular suicide that typically requires new RNA and protein synthesis and is associated with distinct morphological changes including cell shrinkage, cytoplasm membrane blebbing, nuclear fragmentation and DNA degradation. The Bcl-2 gene was first found in t(14:18) containing follicular B-cell lymphomas. A high proportion of these lymphomas contains t(14:18) chromosomal translocations involving the human Bcl-2 gene. Translocation of Bcl-2 sequences from chromosome 18 onto the transcriptionally active immunoglobulin locus at chromosome band 14q32 in B-cells deregulates Bcl-2 gene expression, resulting in high levels of Bcl-2 mRNA and protein expression. Because Bcl-2 blocks apoptosis it may contribute to tumorigenesis by prolonged cell survival rather than by accelerating the rate of cell proliferation. The reduced molecular weight of Bcl-2 is 26 kDa. Additional minor bands at 27-31 kDa and 18-21 kDa may also be observed.
分子量 26 kDa
研究领域 Cancer, Apoptosis/Necrosis
途径 MAPK Pathway, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
说明

Related Products: ABIN968537

限制 仅限研究用
状态 Liquid
浓度 0.25 mg/mL
缓冲液 Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 -20 °C
储存方法 Store both the hamster anti-human Bcl-2 antibody and the Jurkat cell lysate undiluted at -20°C.
厂商提供的图像
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 2 (BCL2) antibody (ABIN967311) Western blot analysis of Bcl-2. A Jurkat cell lysate (Human T-cell leukemia, ATCC TIB...
背景 Núñez, Merino, Grillot, González-García: "Bcl-2 and Bcl-x: regulatory switches for lymphoid death and survival." in: Immunology today, Vol. 15, Issue 12, pp. 582-8, 1995 (PubMed).

Yang, Zha, Jockel, Boise, Thompson, Korsmeyer: "Bad, a heterodimeric partner for Bcl-XL and Bcl-2, displaces Bax and promotes cell death." in: Cell, Vol. 80, Issue 2, pp. 285-91, 1995 (PubMed).

Chiou, Rao, White: "Bcl-2 blocks p53-dependent apoptosis." in: Molecular and cellular biology, Vol. 14, Issue 4, pp. 2556-63, 1994 (PubMed).

Nguyen, Millar, Yong, Korsmeyer, Shore: "Targeting of Bcl-2 to the mitochondrial outer membrane by a COOH-terminal signal anchor sequence." in: The Journal of biological chemistry, Vol. 268, Issue 34, pp. 25265-8, 1994 (PubMed).

Yin, Oltvai, Korsmeyer: "BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax." in: Nature, Vol. 369, Issue 6478, pp. 321-3, 1994 (PubMed).

Batistatou, Merry, Korsmeyer, Greene: "Bcl-2 affects survival but not neuronal differentiation of PC12 cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 13, Issue 10, pp. 4422-8, 1993 (PubMed).

Hockenbery, Oltvai, Yin, Milliman, Korsmeyer: "Bcl-2 functions in an antioxidant pathway to prevent apoptosis." in: Cell, Vol. 75, Issue 2, pp. 241-51, 1993 (PubMed).

Martin, Veis, Korsmeyer, Sugden: "Latent membrane protein of Epstein-Barr virus induces cellular phenotypes independently of expression of Bcl-2." in: Journal of virology, Vol. 67, Issue 9, pp. 5269-78, 1993 (PubMed).

Oltvai, Milliman, Korsmeyer: "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death." in: Cell, Vol. 74, Issue 4, pp. 609-19, 1993 (PubMed).

Veis, Sentman, Bach, Korsmeyer: "Expression of the Bcl-2 protein in murine and human thymocytes and in peripheral T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2546-54, 1993 (PubMed).

Hockenbery, Nuñez, Milliman, Schreiber, Korsmeyer: "Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death." in: Nature, Vol. 348, Issue 6299, pp. 334-6, 1991 (PubMed).

Hockenbery, Zutter, Hickey, Nahm, Korsmeyer: "BCL2 protein is topographically restricted in tissues characterized by apoptotic cell death." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 16, pp. 6961-5, 1991 (PubMed).

McDonnell, Nunez, Platt, Hockenberry, London, McKearn, Korsmeyer: "Deregulated Bcl-2-immunoglobulin transgene expands a resting but responsive immunoglobulin M and D-expressing B-cell population." in: Molecular and cellular biology, Vol. 10, Issue 5, pp. 1901-7, 1990 (PubMed).

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