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Cytokeratin 7 抗体

There are 3+ publications for this product available. The 小鼠 单克隆 anti-Cytokeratin 7 antibody is suitable to detect Cytokeratin 7 in samples from 人, 大鼠, 小鼠, 犬, Pig 和 Hamster. It has been validated for WB 和 IHC.
产品编号 ABIN967289
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Cytokeratin 7 抗体 (ABIN967289)

抗原

See all Cytokeratin 7 (KRT7) 抗体
Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

适用

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人, 大鼠, 小鼠, 犬, Pig, Hamster

宿主

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小鼠

克隆类型

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单克隆

标记

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This Cytokeratin 7 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)

克隆位点

RCK105
  • 交叉反应

    小鼠, 大鼠, Hamster, 犬, 猪

    产品特性

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

    纯化方法

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

    免疫原

    Cytokeratins from the human bladder carcinoma cell line T24

    亚型

    IgG1
  • 应用备注

    Applications include western blot analysis. Additional applications include immunohistochemistry on methanol fixed tissue, but this application is not tested.

    说明

    Related Products: ABIN968535, ABIN967389

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Aqueous buffered solution containing ≤0.09 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store undiluted at 4°C.
  • Smedts, Ramaekers, Robben, Pruszczynski, van Muijen, Lane, Leigh, Vooijs: "Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia." in: The American journal of pathology, Vol. 136, Issue 3, pp. 657-68, (1990) (PubMed).

    Broers, Ramaekers, Rot, Oostendorp, Huysmans, van Muijen, Wagenaar, Vooijs: "Cytokeratins in different types of human lung cancer as monitored by chain-specific monoclonal antibodies." in: Cancer research, Vol. 48, Issue 11, pp. 3221-9, (1988) (PubMed).

    Ramaekers, Huysmans, Schaart, Moesker, Vooijs: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, (1987) (PubMed).

  • 抗原

    Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

    别名

    Cytokeratin 7

    背景

    Intermediate filaments (IF) are a subset of cytoskeletal proteins which function to give overall structural integrity to the plasma membrane as well as organize cells into specific tissues. IF proteins can be divided into six major types based upon similarity in sequence. Type I and II IF proteins are designated keratins based upon whether they are acidic (I) or basic (II) and are characterized by their remarkable biochemical diversity. A subgroup of keratins, designated cytokeratins, are represented in epithelial tissues by at least 20 different polypeptides, designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin 7 is found in both columnar and glandular epithelium of the lung, cervix, breast, bile ducts, and in the collecting ducts in the kidney as well as mesothelium. The molecular weight of cytokeratin 7 is approximately 54 kDa in SDS/PAGE.

    分子量

    55 kDa
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