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GFP 抗体 (N-Term)

GFP 适用: Aequorea victoria IHC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN966201
发货至: 中国
  • 抗原 See all GFP 抗体
    GFP (Green Fluorescent Protein (GFP))
    抗原表位
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    N-Term
    适用
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    Aequorea victoria
    宿主
    • 73
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    克隆类型
    • 120
    • 85
    多克隆
    标记
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    This GFP antibody is un-conjugated
    应用范围
    • 173
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    Immunohistochemistry (IHC)
    纯化方法
    Purified by antigen-specific affinity chromatography.
    免疫原
    Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to N-terminal residues of Aequorea victoria GFP (Green fluorescent protein)
    Top Product
    Discover our top product GFP Primary Antibody
  • 应用备注
    ELISA, Western blotting: 1µg/ml for 2hrs.
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    This antibody is stored in PBS, 50% glycerol
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
  • Inouye, Noguchi, Sakaki, Takagi, Miyata, Iwanaga, Miyata, Tsuji: "Cloning and sequence analysis of cDNA for the luminescent protein aequorin." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 10, pp. 3154-8, (1985) (PubMed).

  • 抗原
    GFP (Green Fluorescent Protein (GFP))
    别名
    GFP (GFP 产品)
    别名
    green fluorescent protein antibody, gfp antibody
    物质类
    Viral Protein
    背景
    GFP(Green fluorescent protein) is an energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. It contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), and oxidation of Tyr-(N+1) to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
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