RFP 抗体 (Red Fluorescent Protein) (AA 234)

Details for Product anti-RFP Antibody No. ABIN964932, 供应商: Log in to see
抗原
抗原表位
AA 234
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适用
Discosoma
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宿主
小鸡
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克隆类型
多克隆
标记
This RFP antibody is un-conjugated
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应用范围
ELISA, Western Blotting (WB)
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选项
Supplier
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Supplier Product No.
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免疫原 The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Immunogen Type: RecombinantProtein
亚型 IgG
特异性 This product was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above.  Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum.
产品特性 Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, fluorometry and fluorescence activated cell sorting (FACS). This product shows optimal performance by western blot.
纯化方法 affinity purified
过滤 Sterile filtered
别名 RFP (RFP Antibody 摘要)
背景 Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.

Synonyms: DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583.
基因ID 55976617
UniProt Q9U6Y8
研究领域 Tags/Labels
应用备注 Anti-RFP is designed to detect recombinant RFP. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.
说明

Gene Name: DsRed

限制 仅限研究用
状态 Liquid
浓度 1.0 mg/mL
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
有效期 12 months
厂商提供的图像
Western Blotting (WB) image for anti-Red Fluorescent Protein (RFP) (AA 234) antibody (ABIN964932) Western blot of Anti-RFP Antibody. 0.1 µg of RFP was loaded on a 4-20% gel and tranfe...
有引用在: Haller, Hock, Giampazolias, Oberst, Green, Debnath, Ryan, Vousden, Tait: "Ubiquitination and proteasomal degradation of ATG12 regulates its proapoptotic activity." in: Autophagy, Vol. 10, Issue 12, pp. 2269-78, 2015 (PubMed).

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