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V5 Epitope 标记 抗体 (HRP)

ABIN962844 产品详细信息, 供应商: Log in to see
抗原
适用
标记
404
3
3
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3
宿主
小鼠
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135
37
9
5
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3
1
1
克隆类型 (克隆位点)
单克隆 ()
标记
HRP
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11
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2
2
2
1
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应用范围
ELISA, Western Blotting (WB)
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36
28
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9
8
5
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4
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Supplier
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品牌 THE™
免疫原 V5 tag peptide conjugated - KLH
序列 GKPIPNPLLG LDST
克隆位点 4C12E11
亚型 IgG2a
特异性 THETM V5 Tag Antibody [HRP], mAb, Mouse recognizes C-terminal tagged fusion proteins, and it slightly recognizes N-terminal V5 tagged fusion proteins.
产品特性 The THETM V5 Tag Antibody [HRP], mAb, Mouse is THETM V5 Tag Antibody, mAb, Mouse conjugated with Horseradish peroxidase (HRP). The antibody is suitable for detecting the expression level of V5 Tagged fusion proteins.
纯化方法 Protein A affinity column
别名 V5 Tag
物质类 Tag
背景 V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.
研究领域 Tags/Labels
应用备注 Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.1 µg/mL.Western blot: 0.2-1 µg/mL.
限制 仅限研究用
状态 Lyophilized
溶解方式 Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.
缓冲液 PBS, pH 7.4, containing 10 mg/mL BSA and 0.01 % Thimerosal.
储存液 Thimerosal (Merthiolate)
注意事项 This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing cycles.
储存条件 4 °C/-20 °C
储存方法 The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.