V5 Epitope Tag 抗体 (Biotin)
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- 抗原 See all V5 Epitope Tag products
- V5 Epitope Tag
- 适用
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This V5 Epitope Tag antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA
- 品牌
- THE™
- 序列
- GKPIPNPLLG LDST
- 特异性
- THETM V5 Tag Antibody [Biotin], mAb, Mouse recognizes N-terminal and C-terminal V5 tagged fusion proteins.
- 产品特性
- The THETM V5 Tag Antibody [Biotin], mAb, Mouse is THETM V5 Tag Antibody conjugated with biotin. The conjugated antibody is suitable for detecting the expression level of V5 Tagged fusion proteins.
- 纯化方法
- Protein A affinity column
- 免疫原
- V5 tag peptide conjugated - KLH
- 克隆位点
- 4C12E11
- 亚型
- IgG2a
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- 应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.005-0.05 µg/mL
Western blot: 0.2-1 µg/mL - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.
- 缓冲液
- PBS, pH 7.4, containing 10 mg/mL BSA and 0.02 % Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freezing and thawing cycles.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
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- 抗原
- V5 Epitope Tag
- 别名
- V5 Tag (V5 Epitope Tag 产品)
- 物质类
- Tag
- 背景
- V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
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