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Phosphotyrosine 抗体

Name: Phosphotyrosine 抗体
Brand: antibodies-online
SKU: ABIN94452
Availability: OutOfStock

适用: Various Species WB, FACS, ICC 宿主: 小鼠 Monoclonal P-Tyr-01 unconjugated

Anti-Phosphotyrosine purified antibody (clone P-Tyr-01) Specificity Verification by Flow Cytometry Anti-Phosphotyrosine purified antibody (concentration in sample 2 μg/mL, GAM FITC, red-filled histogram) binds specifically to surface phosphotyrosines in EGF treated A431 cells (upper panel), but not to the untreated A431 cells (lower panel).

FACS

Phosphotyrosine pattern in HeLa cells as revealed by P-Tyr-01 antibody-Alexa Fluor ® 488 (green).

2 images

(3 references)

产品编号 ABIN94452

发货至: 中国

Datasheet as PDF

抗原 See all Phosphotyrosine products

Phosphotyrosine
适用
19

16

16

4

1
Various Species
宿主
89

28

1
小鼠
Compatible Secondaries
克隆类型
101

17
单克隆
标记
41

16

10

8

4

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Phosphotyrosine antibody is un-conjugated
应用范围
84

61

40

38

37

27

22

17

13

9

9

7

6

3

2

2

2

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

特异性

The antibody P-Tyr-01 detects tyrosine phosphorylation in activated cells.

交叉反应（详细）

Broad

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

Phosphotyrosine conjugated to bovine serum albumin.

克隆位点

P-Tyr-01

亚型

IgG1
anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal unconjugated

anti-Phosphotyrosine antibody
WB, IP, IF, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal E10 unconjugated

anti-Phosphotyrosine antibody
WB, IP, IHC, FACS, ELISA, ICC 宿主: 兔 Monoclonal RM111 unconjugated

anti-Phosphotyrosine antibody (FITC)
WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal FITC

anti-Phosphotyrosine antibody (Atto 488)
WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal Atto 488

anti-Phosphotyrosine antibody (Atto 594)
WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal Atto 594

anti-Phosphotyrosine antibody (Atto 390)
WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal Atto 390

anti-Phosphotyrosine antibody
WB, ELISA 宿主: 小鼠 Monoclonal unconjugated

anti-Phosphotyrosine antibody
适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal unconjugated

anti-Phosphotyrosine antibody
适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal 3F6 unconjugated

应用备注

Western blotting: Recommended dilution: 1-2 μg/mL. Flow cytometry: Recommended dilution: 2-5 μg/mL. Intracellular staining.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Biebl, Riedl, Buchner: "Hsp90 Co-chaperones Form Plastic Genetic Networks Adapted to Client Maturation." in: Cell reports, Vol. 32, Issue 8, pp. 108063, (2020) (PubMed).

Meraner, Horejsí, Wolpl, Fischer, Stingl, Maurer: "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 4, pp. 2262-71, (2007) (PubMed).

Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, (2002) (PubMed).
抗原

Phosphotyrosine

Abstract

Phosphotyrosine 产品

物质类

Amino Acid

背景

PY