小鼠 anti-牛 IgG (Light Chain) Antibody

ABIN94401 产品详细信息, 供应商: Log in to see
抗原
抗原表位
Light Chain
5028
2578
1931
1069
1007
856
330
299
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
1
适用

2912
2859
2405
1752
1485
796
623
441
381
380
355
340
307
293
208
148
139
114
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
宿主
小鼠
6893
4912
1878
894
703
401
92
54
52
21
9
8
5
5
2
1
1
克隆类型 (克隆位点)
单克隆 ()
应用范围
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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免疫原 Fraction of Bovine immunoglobulin.
克隆位点 IVA285-1
亚型 IgG1
特异性 The antibody IVA285-1 reacts with bovine IgG (light chains). IgG light chains (kappa nad lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.
纯化方法 Purified from ascites by protein-A affinity chromatography.
纯度 > 95 % (by SDS-PAGE)
研究领域 Immunology, Secondary Antibodies
应用备注 Western Blotting: Recommended dilution: 0,5-1 µg/mL, overnight in 4 °C
Positive control and sample preparation: BS (bovine serum), dilution 1: 50 in Laemmli reducing buffer, boiled in water bath for 3 min
Application notes: Strongly reacts with bovine IgG light chains, weakly reacts with IgM.Reducing conditions. SDS-PAGE (12 % separating gel).
Flow Cytometry:

Working concentrations should be determined by the investigator.
限制 仅限研究用
状态 Liquid
浓度 1 mg/mL
缓冲液 Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Do not freeze.
储存条件 4 °C
储存方法 Store at 2-8 °C. Do not use after expiration date stamped on vial label.
厂商提供的图像
Western Blotting (WB) image for Mouse anti-Cow (Bovine) IgG (Light Chain) antibody (ABIN94401) Detection of IgG light chain in reduced samples of Fetal Calf Serum (left lane) and B...
有引用在: Antalíková, Simon, Jankovicová, Horovská, Dusinský, Hluchý: "Monoclonal antibody to the light chain of bovine immunoglobulin." in: Hybridoma (2005), Vol. 25, Issue 5, pp. 309-12, 2006 (PubMed).