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小鼠 anti-人 IgG (Fab Region) Antibody

ABIN94400 产品详细信息, 供应商: Log in to see
抗原
抗原表位
Fab Region
4684
2573
1882
1039
1015
825
305
272
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
1
适用

2780
2759
2347
1724
1436
778
607
426
364
338
330
324
307
290
169
148
126
114
41
29
27
25
12
11
9
8
6
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
宿主
小鼠
6750
4661
1834
811
684
394
88
52
51
9
8
5
5
4
2
1
1
克隆类型 (克隆位点)
单克隆 ()
应用范围
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
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免疫原 Purified human IgG.
克隆位点 4A11
亚型 IgG2b
特异性 The antibody 4A11 reacts with Fab fragment of human IgG.
纯化方法 Purified from ascites by precipitation methods and ion exchange chromatography.
纯度 > 95 % (by SDS-PAGE)
背景 Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
分子量 150 kDa
研究领域 Immunology, Secondary Antibodies
应用备注 Working concentrations should be determined by the investigator.I t is recommended that the user titrates the reagent for use in the particular testing system.

Working concentrations should be determined by the investigator.
限制 仅限研究用
状态 Liquid
浓度 1 mg/mL
缓冲液 Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Do not freeze.
储存条件 4 °C
储存方法 Store at 2-8 °C. Do not use after expiration date stamped on vial label.
厂商提供的图像
Western Blotting (WB) image for 小鼠 anti-人 IgG (Fab Region) Antibody (ABIN94400) Western Blotting analysis (reducing conditions) of human IgG Fab fragment using anti-...
背景 Brinkmann, Heusser: "T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors." in: Cellular immunology, Vol. 152, Issue 2, pp. 323-32, 1994 (PubMed).

Balogh, Merétey, Falus, Bozsóky: "Serological abnormalities in juvenile chronic arthritis: a review of 46 cases." in: Annals of the rheumatic diseases, Vol. 39, Issue 2, pp. 129-34, 1980 (PubMed).

Fuller, Keyser: "Serum immunoglobulins after surgical operation." in: Clinical chemistry, Vol. 21, Issue 6, pp. 667-71, 1975 (PubMed).

Franklin: "Structure and function of immunoglobulins." in: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).